ArticleMolecular biology of the cell2002
RNAi in Dictyostelium: the role of RNA-directed RNA polymerases and double-stranded RNase.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
66 citing papers in PubMed, 178 citations in OpenAlex.
- A nanobody-based degron system for targeted protein knockdown in Dictyostelium discoideum.BMC molecular and cell biology · 2026Article
- Multiple Roles of Apolipoprotein E4 in Oxidative Lipid Metabolism and Ferroptosis During the Pathogenesis of Alzheimer's Disease.Journal of molecular neuroscience : MN · 2024Review
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- Knock-in and precise nucleotide substitution using near-PAMless engineered Cas9 variants in Dictyostelium discoideum.Scientific reports · 2021Article
- CRISPR Toolbox for Genome Editing inFrontiers in cell and developmental biology · 2021Article
- Secondary siRNAs in Plants: Biosynthesis, Various Functions, and Applications in Virology.Frontiers in plant science · 2021Review
- Bioenergetics of theBiomolecules · 2020Article
- Article
- Review
- Article
- Retrotransposon Domestication and Control inFrontiers in microbiology · 2017Review
- Identification of distinct biological functions for four 3'-5' RNA polymerases.Nucleic acids research · 2016Article
- Analysis of the Microprocessor in Dictyostelium: The Role of RbdB, a dsRNA Binding Protein.PLoS genetics · 2016Article
- Diverse Evolutionary Trajectories for Small RNA Biogenesis Genes in the Oomycete Genus Phytophthora.Frontiers in plant science · 2016Article
- Nse1 and Nse4, subunits of the Smc5-Smc6 complex, are involved in Dictyostelium development upon starvation.Development, growth & differentiation · 2015Article
- The ABC transporter, AbcB3, mediates cAMP export in D. discoideum development.Developmental biology · 2015Article
- A host factor supports retrotransposition of the TRE5-A population in Dictyostelium cells by suppressing an Argonaute protein.Mobile DNA · 2015Article
- A Simple Retroelement Based Knock-Down System in Dictyostelium: Further Insights into RNA Interference Mechanisms.PloS one · 2015Article
- Argonaute proteins affect siRNA levels and accumulation of a novel extrachromosomal DNA from the Dictyostelium retrotransposon DIRS-1.The Journal of biological chemistry · 2014Article
- The Dictyostelium discoideum RNA-dependent RNA polymerase RrpC silences the centromeric retrotransposon DIRS-1 post-transcriptionally and is required for the spreading of RNA silencing signals.Nucleic acids research · 2014Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We show that in Dictyostelium discoideum an endogenous gene as well as a transgene can be silenced by introduction of a gene construct that is transcribed into a hairpin RNA. Gene silencing was accompanied by the appearance of sequence-specific RNA about 23mers and seemed to have a limited capacity. The three Dictyostelium homologues of the RNA-directed RNA polymerase (RrpA, RrpB, and DosA) all contain an N-terminal helicase domain homologous to the one in the dicer nuclease, suggesting exon shuffling between RNA-directed RNA polymerase and the dicer homologue. Only the knock-out of rrpA resulted in a loss of the hairpin RNA effect and simultaneously in a loss of detectable about 23mers. However, about 23mers were still generated by the Dictyostelium dsRNase in vitro with extracts from rrpA(-), rrpB(-), and DosA(-) cells. Both RrpA and a target gene were required for production of detectable amounts of about 23mers, suggesting that target sequences are involved in about 23mer amplification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.