ArticleThe Journal of cell biology2002
Drosophila Kelch regulates actin organization via Src64-dependent tyrosine phosphorylation.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 81 citations in OpenAlex.
- Cytokinesis genes regulate ring canal patterning and follicle cell differentiation inbioRxiv : the preprint server for biology · 2026Article
- Ring canals in the larval adipose of Drosophila buffer stress response.The Journal of cell biology · 2026Article
- Bridges under construction: the dynamics of ring canal expansion during Drosophila oogenesis.Developmental biology · 2026Review
- F-actin coordinates spindle morphology and function in Drosophila meiosis.PLoS genetics · 2024Article
- TheiScience · 2022Article
- Re-evaluating the actin-dependence of spectraplakin functions during axon growth and maintenance.Developmental neurobiology · 2022Article
- Uncoupling cell division and cytokinesis during germline development in metazoans.Frontiers in cell and developmental biology · 2022Review
- Precise levels of the Drosophila adaptor protein Dreadlocks maintain the size and stability of germline ring canals.Journal of cell science · 2021Article
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- The Arp2/3 complex and the formin, Diaphanous, are both required to regulate the size of germline ring canals in the developing egg chamber.Developmental biology · 2020Article
- Ecological genomics of adaptation to unpredictability in experimental rotifer populations.Scientific reports · 2019Article
- Proximity labeling reveals novel interactomes in liveDevelopment (Cambridge, England) · 2019Article
- The Misshapen kinase regulates the size and stability of the germline ring canals in the Drosophila egg chamber.Developmental biology · 2018Article
- Article
- Review
- Stay Connected: A Germ Cell Strategy.Trends in genetics : TIG · 2017Review
- Structural and functional characterization of the divergent Entamoeba Src using Src inhibitor-1.Parasites & vectors · 2017Article
- Actin Cytoskeletal Organization in Drosophila Germline Ring Canals Depends on Kelch Function in a Cullin-RING E3 Ligase.Genetics · 2015Article
- Drosophila E-cadherin is required for the maintenance of ring canals anchoring to mechanically withstand tissue growth.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Cooperation of the BTB-Zinc finger protein, Abrupt, with cytoskeletal regulators in Drosophila epithelial tumorigenesis.Biology open · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Drosophila kelch gene encodes a member of a protein superfamily defined by the presence of kelch repeats. In Drosophila, Kelch is required to maintain actin organization in ovarian ring canals. We set out to study the actin cross-linking activity of Kelch and how Kelch function is regulated. Biochemical studies using purified, recombinant Kelch protein showed that full-length Kelch bundles actin filaments, and kelch repeat 5 contains the actin binding site. Two-dimensional electrophoresis demonstrated that Kelch is tyrosine phosphorylated in a src64-dependent pathway. Site-directed mutagenesis determined that tyrosine residue 627 is phosphorylated. A Kelch mutant with tyrosine 627 changed to alanine (KelY627A) rescued the actin disorganization phenotype of kelch mutant ring canals, but failed to produce wild-type ring canals. Electron microscopy demonstrated that phosphorylation of Kelch is critical for the proper morphogenesis of actin during ring canal growth, and presence of the nonphosphorylatable KelY627A protein phenocopied src64 ring canals. KelY627A protein in ring canals also dramatically reduced the rate of actin monomer exchange. The phenotypes caused by src64 mutants and KelY627A expression suggest that a major function of Src64 signaling in the ring canal is the negative regulation of actin cross-linking by Kelch.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.