ArticleInfection and immunity2002
Differential fluorescence induction analysis of Streptococcus pneumoniae identifies genes involved in pathogenesis.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
41 citing papers in PubMed, 99 citations in OpenAlex.
- Dynamic Pneumococcal Genetic Adaptations Support Bacterial Growth and Inflammation during Coinfection with Influenza.Infection and immunity · 2021Article
- Identification and functional analysis of glutamine transporter inJournal of oral microbiology · 2020Article
- Role of Streptococcus pneumoniae OM001 operon in capsular polysaccharide production, virulence and survival in human saliva.PloS one · 2018Article
- New Aspects of the Interplay between Penicillin Binding Proteins,Antimicrobial agents and chemotherapy · 2017Article
- Streptococcus pneumoniae Senses a Human-like Sialic Acid Profile via the Response Regulator CiaR.Cell host & microbe · 2016Article
- A functional genomics catalogue of activated transcription factors during pathogenesis of pneumococcal disease.BMC genomics · 2014Article
- Activity of the response regulator CiaR in mutants of Streptococcus pneumoniae R6 altered in acetyl phosphate production.Frontiers in microbiology · 2014Article
- Identification of genes that contribute to the pathogenesis of invasive pneumococcal disease by in vivo transcriptomic analysis.Infection and immunity · 2012Article
- Identification of a novel pneumococcal vaccine antigen preferentially expressed during meningitis in mice.The Journal of clinical investigation · 2012Article
- Characterization of central carbon metabolism of Streptococcus pneumoniae by isotopologue profiling.The Journal of biological chemistry · 2012Article
- Two DHH subfamily 1 proteins contribute to pneumococcal virulence and confer protection against pneumococcal disease.Infection and immunity · 2011Article
- Impact of glutamine transporters on pneumococcal fitness under infection-related conditions.Infection and immunity · 2011Article
- Bioinformatics annotation of the hypothetical proteins found by omics techniques can help to disclose additional virulence factors.Current microbiology · 2009Review
- Human serum induces streptococcal c5a peptidase expression.Infection and immunity · 2009Article
- Opposite effects of Mn2+ and Zn2+ on PsaR-mediated expression of the virulence genes pcpA, prtA, and psaBCA of Streptococcus pneumoniae.Journal of bacteriology · 2008Article
- The role of complex carbohydrate catabolism in the pathogenesis of invasive streptococci.Trends in microbiology · 2008Review
- Photorhabdus luminescens genes induced upon insect infection.BMC genomics · 2008Article
- The influence of in vitro fitness defects on pneumococcal ability to colonize and to cause invasive disease.BMC microbiology · 2008Article
- The utility of affinity-tags for detection of a streptococcal protein from a variety of streptococcal species.Journal of microbiological methods · 2008Article
- Phylogenomic analysis of natural selection pressure in Streptococcus genomes.BMC evolutionary biology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Differential fluorescence induction (DFI) technology was used to identify promoters of Streptococcus pneumoniae induced under various in vitro and in vivo conditions. A promoter-trap library using green fluorescent protein as the reporter was constructed in S. pneumoniae, and the entire library was screened for clones exhibiting increased gfp expression under the chosen conditions. The in vitro conditions used were chosen to mimic aspects of the in vivo environment encountered by the pathogen once it enters a host: changes in temperature, osmolarity, oxygen, and iron concentration, as well as blood. In addition, the library was used to infect animals in three different models, and clones induced in these environments were identified. Several promoters were identified in multiple screens, and genes whose promoters were induced twofold or greater under the inducing condition were mutated to assess their roles in virulence. A total of 25 genes were mutated, and the effects of the mutations were assessed in at least two different infection models. Over 50% of these mutants were attenuated in at least one infection model. We show that DFI is a useful tool for identifying bacterial virulence factors as well as a means of elucidating the microenvironment encountered by pathogens upon infection.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.