ArticleThe Journal of physiology2002
Temperature effect on isometric tension is mediated by regulatory proteins tropomyosin and troponin in bovine myocardium.
Article in The Journal of physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 30 citations in OpenAlex.
- The elementary step that generates force and sinusoidal analysis in striated muscle fibers.Journal of muscle research and cell motility · 2025Review
- Enhancing diastolic function by strain-dependent detachment of cardiac myosin crossbridges.The Journal of general physiology · 2020Article
- Microscopic heat pulses activate cardiac thin filaments.The Journal of general physiology · 2019Article
- Nebulin increases thin filament stiffness and force per cross-bridge in slow-twitch soleus muscle fibers.The Journal of general physiology · 2018Article
- Diversity and similarity of motor function and cross-bridge kinetics in papillary muscles of transgenic mice carrying myosin regulatory light chain mutations D166V and R58Q.Journal of molecular and cellular cardiology · 2013Article
- A study of tropomyosin's role in cardiac function and disease using thin-filament reconstituted myocardium.Journal of muscle research and cell motility · 2013Review
- Micromechanical thermal assays of Ca2+-regulated thin-filament function and modulation by hypertrophic cardiomyopathy mutants of human cardiac troponin.Journal of biomedicine & biotechnology · 2012Article
- Article
- Thin filament-reconstituted skinned muscle fibers for the study of muscle physiology.Journal of biomedicine & biotechnology · 2011Review
- Push and pull of tropomyosin's opposite effects on myosin attachment to actin. A chimeric tropomyosin host-guest study.Biochemistry · 2010Article
- The role of tropomyosin isoforms and phosphorylation in force generation in thin-filament reconstituted bovine cardiac muscle fibres.Journal of muscle research and cell motility · 2010Article
- Tropomyosin period 3 is essential for enhancement of isometric tension in thin filament-reconstituted bovine myocardium.Journal of biophysics (Hindawi Publishing Corporation : Online) · 2009Article
- Impact of temperature on cross-bridge cycling kinetics in rat myocardium.The Journal of physiology · 2007Article
- Temperature-dependence of isometric tension and cross-bridge kinetics of cardiac muscle fibers reconstituted with a tropomyosin internal deletion mutant.Biophysical journal · 2006Article
- X-ray diffraction studies of the thick filament in permeabilized myocardium from rabbit.Biophysical journal · 2006Article
- Temperature change does not affect force between regulated actin filaments and heavy meromyosin in single-molecule experiments.The Journal of physiology · 2006Article
- A quantitative analysis of cardiac myocyte relaxation: a simulation study.Biophysical journal · 2006Article
- Use of thin filament reconstituted muscle fibres to probe the mechanism of force generation.Journal of muscle research and cell motility · 2006Review
- Different effects of cardiac versus skeletal muscle regulatory proteins on in vitro measures of actin filament speed and force.The Journal of physiology · 2005Article
- Role of the N-terminal negative charges of actin in force generation and cross-bridge kinetics in reconstituted bovine cardiac muscle fibres.The Journal of physiology · 2005Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effect of temperature on isometric tension with and without the regulatory proteins tropomyosin and troponin was studied in bovine myocardium using a thin filament removal and reconstitution protocol. In control bovine myocardium, isometric tension increased linearly with temperature in the range 5-40 degrees C: isometric tension at 10 and 30 degrees C was 0.65 and 1.28 times that at 20 degrees C, respectively, with a Q10 of about 1.4. In actin filament-reconstituted myocardium without regulatory proteins, the temperature effect on isometric tension was less: isometric tension at 10 and 30 degrees C was 0.96 and 1.17 times that at 20 degrees C, respectively, with a Q10 of about 1.1. The temperature dependence of the apparent rate constants was studied using sinusoidal analysis. The temperature dependence of 2pi(b) (rate constant of delayed tension phase) did not vary significantly with the regulatory proteins under the standard activating condition (5 mM MgATP, 8 mM P(i), 200 mM ionic strength, pCa 4.66, pH 7.00). Q10 for 2pi(b) in control and actin filament-reconstituted myocardium was 3.8 and 4.0, respectively. There were two phases to the temperature dependence of 2pi(c) (rate constant of quick recovery). In control and thin filament-reconstituted myocardium, Q10 for 2pi(c) was approximately 5.5 in the low temperature range (< or = 25 degrees C) and 2.7 in the high temperature range (> or = 30 degrees C). In actin filament-reconstituted myocardium, Q10 for 2pi(c) was 8.5 in the low temperature range and 3.6 in the high temperature range. The above results demonstrate that regulatory proteins augment the temperature dependence of isometric tension, indicating that the regulatory proteins may modify the actomyosin interaction.
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