ArticleThe Journal of physiology2002
Basolateral PAR-2 receptors mediate KCl secretion and inhibition of Na+ absorption in the mouse distal colon.
Article in The Journal of physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 62 citations in OpenAlex.
- Measurement of novel intestinal secretory and barrier pathways and effects of proteases.Neurogastroenterology and motility · 2019Review
- Protease-Activated Receptors in the Intestine: Focus on Inflammation and Cancer.Frontiers in endocrinology · 2019Review
- Protease-activated receptors in kidney disease progression.American journal of physiology. Renal physiology · 2016Review
- Carbachol-induced colonic mucus formation requires transport via NKCC1, K⁺ channels and CFTR.Pflugers Archiv : European journal of physiology · 2015Article
- Luminal trypsin induces enteric nerve-mediated anion secretion in the mouse cecum.The journal of physiological sciences : JPS · 2014Article
- Renal proteinase-activated receptor 2, a new actor in the control of blood pressure and plasma potassium level.The Journal of biological chemistry · 2013Article
- Enteric bacterial proteases in inflammatory bowel disease- pathophysiology and clinical implications.World journal of gastroenterology · 2013Review
- Proteolytic activation of the epithelial sodium channel (ENaC) by the cysteine protease cathepsin-S.Pflugers Archiv : European journal of physiology · 2012Article
- Subepithelial trypsin induces enteric nerve-mediated anion secretion by activating proteinase-activated receptor 1 in the mouse cecum.The journal of physiological sciences : JPS · 2012Article
- Lesioning of TRPV1 expressing primary afferent neurons prevents PAR-2 induced motility, but not mechanical hypersensitivity in the rat colon.Neurogastroenterology and motility · 2012Article
- Apical and basolateral pools of proteinase-activated receptor-2 direct distinct signaling events in the intestinal epithelium.American journal of physiology. Cell physiology · 2011Article
- Role of enteric nerves in immune-mediated changes in protease-activated receptor 2 effects on gut function.Neurogastroenterology and motility · 2010Article
- Proteinase-activated receptor 2 stimulates Na,K-ATPase and sodium reabsorption in native kidney epithelium.The Journal of biological chemistry · 2008Article
- Gastrointestinal roles for proteinase-activated receptors in health and disease.British journal of pharmacology · 2008Review
- Proteinases and signalling: pathophysiological and therapeutic implications via PARs and more.British journal of pharmacology · 2008Review
- Aldosterone responsiveness of the epithelial sodium channel (ENaC) in colon is increased in a mouse model for Liddle's syndrome.The Journal of physiology · 2008Article
- Protease-activated receptor 2, dipeptidyl peptidase I, and proteases mediate Clostridium difficile toxin A enteritis.Gastroenterology · 2007Article
- Role for protease activity in visceral pain in irritable bowel syndrome.The Journal of clinical investigation · 2007Article
- Suppression of pancreatitis-related allodynia/hyperalgesia by proteinase-activated receptor-2 in mice.British journal of pharmacology · 2006Article
- Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Proteinase-activated receptor-2 (PAR-2) may participate in epithelial ion transport regulation. Here we examined the effect of mouse activating peptide (mAP), a specific activator of PAR-2, on electrogenic transport of mouse distal colon using short-circuit current (I(SC)) measurements. Under steady-state conditions, apical application of amiloride (100 microM) revealed a positive I(SC) component of 74.3 +/- 6.8 microA x cm(-2) indicating the presence of Na+ absorption, while apical Ba2+ (10 mM) identified a negative I(SC) component of 26.2 +/- 1.8 microA x cm(-2) consistent with K+ secretion. Baseline Cl- secretion was minimal. Basolateral addition of 20 microM mAP produced a biphasic I(SC) response with an initial transient peak increase of 11.2 +/- 0.9 microA x cm(-2), followed by a sustained fall to a level 31.2 +/- 2.6 microA x cm(-2) (n = 43) below resting I(SC). The peak response was due to Cl- secretion as it was preserved in the presence of amiloride but was largely reduced in the presence of basolateral bumetanide (20 microM) or in the absence of extracellular Cl-. The secondary decline of I(SC) was also attenuated by bumetanide and by Ba2+, indicating that it is partly due to a stimulation of K+ secretion. In addition, the amiloride-sensitive I(SC) was slightly reduced by mAP, suggesting that inhibition of Na+ absorption also contributes to the I(SC) decline. Expression of PAR-2 in mouse distal colon was confirmed using RT-PCR and immunocytochemistry. We conclude that functional basolateral PAR-2 is present in mouse distal colon and that its activation stimulates Cl- and K+ secretion while inhibiting baseline Na+ absorption.
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