ArticleThe Journal of physiology2002
Comparison of the properties of CLCA1 generated currents and I(Cl(Ca)) in murine portal vein smooth muscle cells.
Article in The Journal of physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 54 citations in OpenAlex.
- Polymodal Control of TMEM16x Channels and Scramblases.International journal of molecular sciences · 2022Review
- Arrhythmogenic Remodeling in the Failing Heart.Cells · 2021Review
- Secreted CLCA1 modulates TMEM16A to activate Ca(2+)-dependent chloride currents in human cells.eLife · 2015Article
- Phenomics of cardiac chloride channels.Comprehensive Physiology · 2013Review
- Potassium and ANO1/ TMEM16A chloride channel profiles distinguish atypical and typical smooth muscle cells from interstitial cells in the mouse renal pelvis.British journal of pharmacology · 2012Article
- Expression profile and protein translation of TMEM16A in murine smooth muscle.American journal of physiology. Cell physiology · 2010Article
- Cholesterol depletion alters amplitude and pharmacology of vascular calcium-activated chloride channels.Cardiovascular research · 2010Article
- TMEM16A/anoctamin 1 protein mediates calcium-activated chloride currents in pulmonary arterial smooth muscle cells.The Journal of physiology · 2010Article
- Inhibition of vascular calcium-gated chloride currents by blockers of KCa1.1, but not by modulators of KCa2.1 or KCa2.3 channels.British journal of pharmacology · 2009Article
- Human ClCa1 modulates anionic conduction of calcium-dependent chloride currents.The Journal of physiology · 2009Article
- Phenomics of cardiac chloride channels: the systematic study of chloride channel function in the heart.The Journal of physiology · 2009Review
- Expression, localization, and functional properties of Bestrophin 3 channel isolated from mouse heart.American journal of physiology. Cell physiology · 2008Article
- Pharmacological and molecular evidence for the involvement of Kv4.3 in ultra-fast activating K+ currents in murine portal vein myocytes.British journal of pharmacology · 2006Article
- Mechanism of the inhibition of Ca2+-activated Cl- currents by phosphorylation in pulmonary arterial smooth muscle cells.The Journal of general physiology · 2006Article
- Diversity of Cl(-) channels.Cellular and molecular life sciences : CMLS · 2006Review
- Distribution of cGMP-dependent and cGMP-independent Ca(2+)-activated Cl(-) conductances in smooth muscle cells from different vascular beds and colon.Pflugers Archiv : European journal of physiology · 2005Article
- Electrophysiological and functional effects of the KCNQ channel blocker XE991 on murine portal vein smooth muscle cells.British journal of pharmacology · 2005Article
- Ca(2+)- and volume-sensitive chloride currents are differentially regulated by agonists and store-operated Ca2+ entry.The Journal of general physiology · 2005Article
- Electrophysiological characterization and functional importance of calcium-activated chloride channel in rat uterine myocytes.Pflugers Archiv : European journal of physiology · 2004Article
- Single cGMP-activated Ca(+)-dependent Cl(-) channels in rat mesenteric artery smooth muscle cells.The Journal of physiology · 2004Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
Calcium-activated chloride currents (I(Cl(Ca))) have been recorded in various smooth muscle cells but, to date, there has been no information as to the molecular nature of the channel underlying this conductance. We have characterised native I(Cl(Ca)) in freshly dispersed smooth muscle cells isolated from murine portal vein using whole-cell voltage clamp. I(Cl(Ca)) exhibited time-dependent activation at depolarised potentials and rapid deactivation upon repolarisation. The reversal potential of I(Cl(Ca)) was close to the theoretical equilibrium potential (E(Cl)) and was shifted by replacement of external Cl- by SCN- or isethionate. Dithiothreitol (DTT, 1 mM), a blocker of CLCA1, had no effect on the I(Cl(Ca)) current in myocytes. RT-PCR demonstrated the expression of mCLCA1 transcripts, but not mCLCA3 transcripts, in various murine smooth muscle cells including portal vein, as well as cardiomyocytes, and the levels of mCLCA1 transcriptional expression were quantified by real time quantitative RT-PCR. Stable transfection of HEK293 cells with the cDNA encoding mCLCA1 cloned from murine portal vein smooth muscle yielded a current with notable differences in Ca2+ sensitivity, channel kinetics and modulation by DTT from the native I(Cl(Ca)). However, there was some similarity in the pore properties and these data suggest that mCLCA1 alone does not comprise the Cl- channel in portal vein smooth muscle cells.
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