ArticleProtein science : a publication of the Protein Society2002
Detecting equilibrium cytochrome c folding intermediates by electrospray ionisation mass spectrometry: two partially folded forms populate the molten-globule state.
Article in Protein science : a publication of the Protein Society, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 91 citations in OpenAlex.
- Differences in α-synuclein conformational states in physiologically relevant pH/NaThe Analyst · 2026Article
- Insights into Fluctuations of Structure of Proteins: Significance of Intermediary States in Regulating Biological Functions.Polymers · 2022Review
- pH Dependence of the Number of Discrete Conformers of Carbonic Anhydrase 2, as Evaluated from Collision Cross-Section Using Ion Mobility Coupled with Electrospray Ionization.Mass spectrometry (Tokyo, Japan) · 2018Article
- Addressing a Common Misconception: Ammonium Acetate as Neutral pH "Buffer" for Native Electrospray Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2017Article
- Insights into the mechanism of protein electrospray ionization from salt adduction measurements.Journal of the American Society for Mass Spectrometry · 2014Article
- Mass spectrometry-based methods to study protein architecture and dynamics.Protein science : a publication of the Protein Society · 2013Review
- The pH Dependence of Product Ion Spectra Obtained from Precursor Ions with the Same Charge Number in ESI of Carbonic Anhydrase 2.Mass spectrometry (Tokyo, Japan) · 2013Article
- Vapor treatment of electrospray droplets: evidence for the folding of initially denatured proteins on the sub-millisecond time-scale.Journal of the American Society for Mass Spectrometry · 2012Article
- Advanced mass spectrometry-based methods for the analysis of conformational integrity of biopharmaceutical products.Current pharmaceutical biotechnology · 2011Review
- Protein-protein binding affinities in solution determined by electrospray mass spectrometry.Journal of the American Society for Mass Spectrometry · 2011Article
- Electrospray droplet exposure to gaseous acids for the manipulation of protein charge state distributions.Analytical chemistry · 2010Article
- Article
- Discrimination among IgG1-kappa monoclonal antibodies produced by two cell lines using charge state distributions in nanoESI-TOF mass spectra.Journal of the American Society for Mass Spectrometry · 2009Article
- Irreversible thermal denaturation of cytochrome C studied by electrospray mass spectrometry.Journal of the American Society for Mass Spectrometry · 2009Article
- Monitoring copopulated conformational states during protein folding events using electrospray ionization-ion mobility spectrometry-mass spectrometry.Journal of the American Society for Mass Spectrometry · 2007Article
- Analytical performance of a venturi-assisted array of micromachined ultrasonic electrosprays coupled to ion trap mass spectrometry for the analysis of peptides and proteins.Analytical chemistry · 2007Article
- The first step of hen egg white lysozyme fibrillation, irreversible partial unfolding, is a two-state transition.Protein science : a publication of the Protein Society · 2007Article
- Direct correlation of the crystal structure of proteins with the maximum positive and negative charge states of gaseous protein ions produced by electrospray ionization.Journal of the American Society for Mass Spectrometry · 2005Article
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nanoelectrospray ionization mass spectrometry (nano-ESI-MS) is applied to the characterization of ferric cytochromec (cytc) conformational states under different solvent conditions. The methanol-induced molten-globule state in the pH range 2.6-3.0 is found to be populated by two distinct, partially folded conformers I(A) and I(B). The more compact intermediate I(B) resembles that induced by glycerol in acid-unfolded cytc. The less compact one, I(A), also can be induced by destabilization of the native structure by trifluoroethanol. I(A) and I(B) can be detected, in the absence of additives, around the midpoint of the acid-induced unfolding transition, providing direct evidence for involvement of equilibrium folding intermediates in cytc conformational transitions at low pH. This study shows that mass spectrometry can contribute to the characterization of molten-globule states of proteins by detection of distinct, although poorly populated, conformations involved in a dynamic equilibrium.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.