ArticleThe EMBO journal2002
A chloroplast protein binds a viroid RNA in vivo and facilitates its hammerhead-mediated self-cleavage.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 124 citations in OpenAlex.
- Derailing the host machinery to achieve replication: how viroid and viroid-like RNAs successfully copy their genomes in hostile territory.RNA biology · 2025Review
- Eggplant latent viroid is located in the chloroplasts and nuclei of eggplant infected cells.Virology journal · 2024Article
- Understanding viroids, endogenous circular RNAs, and viroid-like RNAs in the context of biogenesis.PLoS pathogens · 2024Article
- Cellular roadmaps of viroid infection.Trends in microbiology · 2023Review
- Viroids: Non-Coding Circular RNAs Able to Autonomously Replicate and Infect Higher Plants.Biology · 2023Review
- Article
- Transcriptomics Advancement in the Complex Response of Plants to Viroid Infection.International journal of molecular sciences · 2022Review
- Article
- Current view and perspectives in viroid replication.Current opinion in virology · 2021Review
- An Inside Look into Biological Miniatures: Molecular Mechanisms of Viroids.International journal of molecular sciences · 2021Review
- Novel ribozymes: discovery, catalytic mechanisms, and the quest to understand biological function.Nucleic acids research · 2019Article
- Combining a Simple Method for DNA/RNA/Protein Co-Purification and Arabidopsis Protoplast Assay to Facilitate Viroid Research.Viruses · 2019Article
- Article
- The Role of the Chloroplast in the Replication of Positive-Sense Single-Stranded Plant RNA Viruses.Frontiers in plant science · 2018Article
- NF90-NF45 is a selective RNA chaperone that rearranges viral and cellular riboswitches: biochemical analysis of a virus host factor activity.Nucleic acids research · 2017Article
- Top-down/Bottom-up Mass Spectrometry Workflow Using Dissolvable Polyacrylamide Gels.Analytical chemistry · 2017Article
- Article
- Eggplant latent viroid: a friendly experimental system in the family Avsunviroidae.Molecular plant pathology · 2016Review
- Elimination of chrysanthemum stunt viroid and chrysanthemum chlorotic mottle viroid from infected chrysanthemum by cryopreservation.Protoplasma · 2016Article
- Engineering viroid resistance.Viruses · 2015Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viroids, small single-stranded circular RNAs (246-401 nucleotides), do not have mRNA capacity and must recruit host proteins to assist in the steps of their biological cycle. The nature of these cellular factors is poorly understood due to a lack of reliable experimental approaches. Here, to screen for host proteins interacting with viroid RNAs in vivo, we UV-irradiated avocado leaves infected with avocado sunblotch viroid (ASBVd), the type member of chloroplast viroids containing hammerhead ribozymes. This resulted in the detection of several ASBVd-host protein adducts. Tandem mass spectrometry analysis of the most abundant cross-linked species identified the protein component as two closely related chloroplast RNA-binding proteins (PARBP33 and PARBP35) of a family whose members previously have been shown to be involved in stabilization, maturation and editing of chloroplast transcripts. PARBP33 behaves as an RNA chaperone that stimulates in vitro the hammerhead-mediated self-cleavage of the multimeric ASBVd transcripts that result from rolling circle replication, indicating that this reaction, despite its RNA-based mechanism, is facilitated by proteins. The structural and functional parallelism between PARBP33 and PARBP35, and some proteins involved in viral RNA replication, indicates that viroids and RNA viruses recruit similar host proteins for their replication.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.