ArticleThe EMBO journal2002
Membrane sequestration of the signal transduction protein GlnK by the ammonium transporter AmtB.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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Who cites it
85 citing papers in PubMed, 234 citations in OpenAlex.
- Bacterial sensing and response for neutralization and detoxification of environmental ammonia.Journal of bacteriology · 2026Article
- A Key Metabolic Protein in Active Mycobacterium tuberculosis: Insights into Carbon, Nitrogen, and Sulfur Metabolism.Advances in experimental medicine and biology · 2026Review
- The Molecular Basis of Amino Acids Sensing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Deciphering the ammonia transformation mechanism of a novel marine multi-stress-tolerant yeast,Applied and environmental microbiology · 2025Article
- Transcriptome analysis of nitrogen assimilation preferences inFrontiers in microbiology · 2025Article
- Article
- The PII protein interacts with the Amt ammonium transport and modulates nitrate/nitrite assimilation in mycobacteria.Frontiers in microbiology · 2024Article
- Flipping the switch: dynamic modulation of membrane transporter activity in bacteria.Microbiology (Reading, England) · 2023Review
- Prokaryotic ammonium transporters: what has three decades of research revealed?Microbiology (Reading, England) · 2023Article
- Comparative Genomics Reveals Genetic Diversity and Metabolic Potentials of the GenusMicrobiology spectrum · 2022Article
- Gene Fitness of Azotobacter vinelandii under Diazotrophic Growth.Journal of bacteriology · 2021Article
- Quasi-essentiality of RNase Y in Bacillus subtilis is caused by its critical role in the control of mRNA homeostasis.Nucleic acids research · 2021Article
- Multi-omics Study of Planobispora rosea, Producer of the Thiopeptide Antibiotic GE2270A.mSystems · 2021Article
- Rapid Response of Nitrogen Cycling Gene Transcription to Labile Carbon Amendments in a Soil Microbial Community.mSystems · 2021Article
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- Identification and functional analysis of glutamine transporter inJournal of oral microbiology · 2020Article
- NADThe Journal of biological chemistry · 2020Article
- Nutrient Limitation Causes Differential Expression of Transport- and Metabolism Genes in the Compartmentalized Anammox BacteriumFrontiers in microbiology · 2020Article
- Parallel experimental evolution reveals a novel repressive control of GalP on xylose fermentation in Escherichia coli.Biotechnology and bioengineering · 2019Article
- Sustained sensing in potassium homeostasis: Cyclic di-AMP controls potassium uptake by KimA at the levels of expression and activity.The Journal of biological chemistry · 2019Article
25 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Amt proteins are ammonium transporters that are conserved throughout all domains of life, being found in bacteria, archaea and eukarya. In bacteria and archaea, the Amt structural genes (amtB) are invariably linked to glnK, which encodes a member of the P(II) signal transduction protein family, proteins that regulate enzyme activity and gene expression in response to the intracellular nitrogen status. We have now shown that in Escherichia coli and Azotobacter vinelandii, GlnK binds to the membrane in an AmtB-dependent manner and that GlnK acts as a negative regulator of the transport activity of AmtB. Membrane binding is dependent on the uridylylation state of GlnK and is modulated according to the cellular nitrogen status such that it is maximal in nitrogen-sufficient situations. The membrane sequestration of GlnK by AmtB represents a novel form of signal transduction in which an integral membrane transport protein functions to link the extracellular ammonium concentration to the intracellular responses to nitrogen status. The results also offer new insights into the evolution of P(II) proteins and a rationale for their trigonal symmetry.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.