ArticleJournal of bacteriology2002
Trinucleotide GAA repeats dictate pMGA gene expression in Mycoplasma gallisepticum by affecting spacing between flanking regions.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- Designing of novel chimeric PvpA-pMGA protein of Mycoplasma gallisepticum, applicable for indirect ELISA.Journal, genetic engineering & biotechnology · 2022Article
- Current status of vaccine research, development, and challenges of vaccines for Mycoplasma gallisepticum.Poultry science · 2020Review
- Comparative Analysis ofFrontiers in genetics · 2018Article
- Article
- Global Changes in Mycoplasma gallisepticum Phase-Variable Lipoprotein Gene vlhA Expression during In Vivo Infection of the Natural Chicken Host.Infection and immunity · 2016Article
- Reconstruction of Transcription Control Networks in Mollicutes by High-Throughput Identification of Promoters.Frontiers in microbiology · 2016Article
- A repetitive DNA element regulates expression of the Helicobacter pylori sialic acid binding adhesin by a rheostat-like mechanism.PLoS pathogens · 2014Article
- Identification of lipoprotein MslA as a neoteric virulence factor of Mycoplasma gallisepticum.Infection and immunity · 2010Article
- A novel phase variation mechanism in the meningococcus driven by a ligand-responsive repressor and differential spacing of distal promoter elements.PLoS pathogens · 2009Article
- A conserved tetranucleotide repeat is necessary for wild-type expression of the Moraxella catarrhalis UspA2 protein.Journal of bacteriology · 2006Article
- Deletions within a CA-repeat-rich region of intron 4 of the human SP-B gene affect mRNA splicing.The Biochemical journal · 2005Article
- Microsatellite instability regulates transcription factor binding and gene expression.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Characterization of a trinucleotide repeat sequence (CGG)5 and potential use in restriction fragment length polymorphism typing of Mycobacterium tuberculosis.Journal of clinical microbiology · 2004Article
- Simple sequence repeats and compositional bias in the bipartite Ralstonia solanacearum GMI1000 genome.BMC genomics · 2003Article
- Phenotypic switching in Mycoplasma gallisepticum hemadsorption is governed by a high-frequency, reversible point mutation.Infection and immunity · 2003Article
- Association tests with systemic lupus erythematosus (SLE) of IL10 markers indicate a direct involvement of a CA repeat in the 5' regulatory region.Genes and immunity · 2002Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The pMGA genes of the avian respiratory pathogen Mycoplasma gallisepticum encode a family of hemagglutinins that are subject to phase variation. A trinucleotide GAA repeat region is located upstream of the pMGA transcription start site. The length of the repeat region varies at a high frequency due to changes in the number of repeat units. Previous studies have shown that pMGA genes are transcribed when 12 GAA repeats are present but are not transcribed when the number of repeats is not 12. To further analyze the mechanism of gene regulation, the pMGA promoter region was modified either by deleting the nucleotides 5" of the GAA repeats or by inserting linkers of 10 or 12 bp at a position 3" of the repeats. The modified promoter region was fused to a promoterless lacZ gene and transformed into M. gallisepticum by using transposon Tn4001 as a vector. Transformants and successive generations of progeny were analyzed with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) to monitor beta-galactosidase activity. For the transformants of M. gallisepticum containing the reporter with deletion of nucleotides 5" of the GAA repeats, GAA-dependent pMGA gene regulation was abolished. For the transformants containing the reporter with an addition of 10- or 12-bp linkers, lacZ was expressed only when eight GAA repeats were present. These data indicate that the nucleotides 5" of the GAA repeats as well as the spacing between the GAA repeats and sequences downstream (3") of the repeats are important for pMGA gene expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.