ArticleJournal of bacteriology2002
Salmonella enterica serovar typhimurium resistance to bile: identification and characterization of the tolQRA cluster.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
48 citing papers in PubMed, 92 citations in OpenAlex.
- How bile acids and the microbiota interact to shape host immunity.Nature reviews. Immunology · 2024Review
- Roles of the Tol/Pal System in Bacterial Pathogenesis and Its Application to Antibacterial Therapy.Vaccines · 2022Review
- The role of TolA, TolB, and TolR in cell morphology, OMVs production, and virulence of Salmonella Choleraesuis.AMB Express · 2022Article
- Effects of 405 ± 5-nm LED Illumination on Environmental Stress Tolerance ofFoods (Basel, Switzerland) · 2022Article
- Phytocompounds from Himalayan Medicinal Plants as Potential Drugs to Treat Multidrug-ResistantBiomedicines · 2021Article
- Gallbladder carriage generates genetic variation and genome degradation in Salmonella Typhi.PLoS pathogens · 2020Article
- A Multi-Scale Approach to Investigate Adhesion Properties ofFoods (Basel, Switzerland) · 2020Article
- The multifarious roles of Tol-Pal in Gram-negative bacteria.FEMS microbiology reviews · 2020Review
- The Salmonella enterica Serovar TyphiJournal of bacteriology · 2020Article
- Twin-Arginine Translocation System Is Involved in Citrobacter rodentium Fitness in the Intestinal Tract.Infection and immunity · 2020Article
- Salmonella enterica Serovar Typhimurium Uses PbgA/YejM To Regulate Lipopolysaccharide Assembly during Bacteremia.Infection and immunity · 2019Article
- Crystal structure of the multidrug resistance regulator RamR complexed with bile acids.Scientific reports · 2019Article
- Contribution of the Cpx envelope stress system to metabolism and virulence regulation in Salmonella enterica serovar Typhimurium.PloS one · 2019Article
- Similarities and Differences between Colicin and Filamentous Phage Uptake by Bacterial Cells.EcoSal Plus · 2019Review
- YraP Contributes to Cell Envelope Integrity and Virulence of Salmonella enterica Serovar Typhimurium.Infection and immunity · 2018Article
- Salmonella Tol-Pal Reduces Outer Membrane Glycerophospholipid Levels for Envelope Homeostasis and Survival during Bacteremia.Infection and immunity · 2018Article
- Plant-made Salmonella bacteriocins salmocins for control of Salmonella pathovars.Scientific reports · 2018Article
- Interactions between Bacteria and Bile Salts in the Gastrointestinal and Hepatobiliary Tracts.Frontiers in medicine · 2017Review
- Association of diverse bacterial communities in human bile samples with biliary tract disorders: a survey using culture and polymerase chain reaction-denaturing gradient gel electrophoresis methods.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Article
- The O-Antigen Capsule of Salmonella enterica Serovar Typhimurium Facilitates Serum Resistance and Surface Expression of FliC.Infection and immunity · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salmonella enterica serovar Typhimurium is resistant to the action of bile salts, and resistance to bile is enhanced in strains in which the PhoP-PhoQ (PhoPQ) two-component regulatory system has been activated. To identify genes necessary for bile resistance, MudJ transposon mutagenesis was performed on a strain containing a phoP mutation that results in constitutive expression of PhoP-activated genes. After screening >10,000 mutants for the loss of growth on Luria-Bertani broth-bile plates, 14 bile-sensitive mutants were identified. Of these 14 mutants, 3 were found to retain the bile sensitivity phenotype upon P22 transduction, to possess wild-type growth characteristics, and to contain a smooth lipopolysaccharide. Southern hybridization experiments showed that all three strains contained unique insertions. DNA sequencing of the transposon-chromosomal-DNA fusion junctions of these strains showed all to be linked to the putative Salmonella orf1-tolQRA operon, with insertions in tolQ, orf1, and a gene upstream of the orf1-tolQRA operon not previously associated with Tol function (orfX). Through the use of transcriptional fusions, none of the putative tol (or tol-associated) genes were shown to be regulated by PhoPQ, bile, or the RcsC-RcsB two-component system; however, all of the genes (orfX, orf1, tolQRA) are predicted to be cotranscribed. This is the first identification of Salmonella serovar Typhimurium Tol homologs and the first demonstration of their role in bile resistance in this organism. In addition, the observed regulation, operon arrangement, and phenotypes associated with these tol genes demonstrate significant differences from their Escherichia coli homologs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.