ArticleProceedings of the National Academy of Sciences of the United States of America2002
Laser capture microdissection analysis of gene expression in macrophages from atherosclerotic lesions of apolipoprotein E-deficient mice.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed, 165 citations in OpenAlex.
- Laser-capture microdissection for spatial transcriptomics of immunohistochemically detected neurons.The Journal of biological chemistry · 2025Article
- Transcriptomic research in atherosclerosis: Unravelling plaque phenotype and overcoming methodological challenges.Journal of molecular and cellular cardiology plus · 2023Review
- Macrophage Anti-inflammatory Behaviour in a Multiphase Model of Atherosclerotic Plaque Development.Bulletin of mathematical biology · 2023Article
- Inflammation Resolution: Implications for Atherosclerosis.Circulation research · 2022Review
- Survey of Approaches for Investigation of Atherosclerosis In Vivo.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Hyperglycemia Induces Trained Immunity in Macrophages and Their Precursors and Promotes Atherosclerosis.Circulation · 2021Article
- Inhibiting LXRα phosphorylation in hematopoietic cells reduces inflammation and attenuates atherosclerosis and obesity in mice.Communications biology · 2021Article
- Wnt signaling enhances macrophage responses to IL-4 and promotes resolution of atherosclerosis.eLife · 2021Article
- Exosome-mediated delivery of inflammation-responsiveTheranostics · 2021Article
- Mutual Interplay of Host Immune System and Gut Microbiota in the Immunopathology of Atherosclerosis.International journal of molecular sciences · 2020Review
- LXRα Phosphorylation in Cardiometabolic Disease: Insight From Mouse Models.Endocrinology · 2020Review
- Neutrophil extracellular traps promote macrophage inflammation and impair atherosclerosis resolution in diabetic mice.JCI insight · 2020Article
- Macrophages in Atherosclerosis Regression.Arteriosclerosis, thrombosis, and vascular biology · 2020Review
- Platelet regulation of myeloid suppressor of cytokine signaling 3 accelerates atherosclerosis.Science translational medicine · 2019Article
- Role of LpL (Lipoprotein Lipase) in Macrophage Polarization In Vitro and In Vivo.Arteriosclerosis, thrombosis, and vascular biology · 2019Article
- Differential Gene Expression in Macrophages From Human Atherosclerotic Plaques Shows Convergence on Pathways Implicated by Genome-Wide Association Study Risk Variants.Arteriosclerosis, thrombosis, and vascular biology · 2018Article
- Macrophage Trafficking, Inflammatory Resolution, and Genomics in Atherosclerosis: JACC Macrophage in CVD Series (Part 2).Journal of the American College of Cardiology · 2018Review
- Atherosclerosis in the single-cell era.Current opinion in lipidology · 2018Review
- Data on leukocyte PDZK1 deficiency affecting macrophage apoptosis but not monocyte recruitment, cell proliferation, macrophage abundance or ER stress in atheroscleroticData in brief · 2018Article
- Reduced Necrosis and Content of Apoptotic M1 Macrophages in Advanced Atherosclerotic Plaques of Mice With Macrophage-Specific Loss of Trpc3.Scientific reports · 2017Article
14 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 1 country.
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Abstract
Macrophage foam cells are integral in the development of atherosclerotic lesions. Gene expression analysis of lesional macrophage foam cells is complicated by the cellular heterogeneity of atherosclerotic plaque and the presence of lesions of various degrees of severity. To overcome these limitations, we tested the ability of laser capture microdissection (LCM) and real-time quantitative reverse transcription PCR to selectively analyze RNA from lesional macrophages of apolipoprotein E (apoE)-deficient mice. Proximal aortic tissue sections were immunostained for macrophagespecific CD68/macrosialin by a rapid (approximately 15-min) protocol. Alternating sections from each animal were used to isolate RNA either from entire sections (analogous to isolation from whole tissue) or by LCM selection of CD68-positive cells. We measured the mRNA levels of CD68, a macrophage-specific marker, alpha-actin, a smooth muscle cell marker, and cyclophilin A, a control gene. Compared with whole sections, CD68 mRNA levels were greatly enriched (33.6-fold) in the laser-captured lesional macrophages. In contrast to whole sections, LCM-derived RNA had undetectable levels of alpha-actin. To illustrate the ability of this method to measure changes in lesional macrophage gene expression, we injected 100 microg of lipopolysaccharide i.p. into apoE-deficient mice and detected in laser-captured lesional macrophages increased mRNA expression for vascular cell adhesion molecule-1, intercellular cell adhesion molecule-1, and monocyte chemoattractant protein-1 (11.9-, 32.5-, and 31.0-fold, respectively). By selectively enriching foam cell RNA, LCM provides a powerful approach to study the in situ expression and regulation of atherosclerosis-related genes. This approach will allow the study of macrophage gene expression under various conditions of plaque formation, regression, and response to genetic and environmental perturbations.
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