ArticlePlant physiology2002
Manipulation of strawberry fruit softening by antisense expression of a pectate lyase gene.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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87 citing papers in PubMed, 358 citations in OpenAlex.
- The impact of 1-MCP treatment combined with polyethylene film bag packaging on the quality of 'Shannong Su' pears during storage.Food chemistry. Molecular sciences · 2026Article
- FaRIF as a key regulator of strawberry fruit ripening: deciphering its targets and interaction networks.Horticulture research · 2026Article
- Advances in Cell Wall Dynamics and Gene Expression in Postharvest Fruit Softening.Plants (Basel, Switzerland) · 2025Review
- Cell-Penetrating Peptide-Mediated Delivery of Gene-Silencing Nucleic Acids to the Invasive Common ReedPlants (Basel, Switzerland) · 2025Article
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- Cotton Pectate Lyase GhPEL48_Dt Promotes Fiber Initiation Mediated by Histone Acetylation.Plants (Basel, Switzerland) · 2024Article
- Genome-Wide Analysis of Polygalacturonase Gene Family Reveals Its Role in Strawberry Softening.Plants (Basel, Switzerland) · 2024Article
- Exploration of a European-centered strawberry diversity panel provides markers and candidate genes for the control of fruit quality traits.Horticulture research · 2024Article
- Insights into the cell-wall dynamics in grapevine berries during ripening and in response to biotic and abiotic stresses.Plant molecular biology · 2024Review
- Examining preharvest genetic and morphological factors contributing to lettuce (Lactuca sativa L.) shelf-life.Scientific reports · 2024Article
- Unraveling cell wall polysaccharides during blueberry ripening: insights into the roles of rhamnogalacturonan-I and arabinogalactan proteins in fruit firmness.Frontiers in plant science · 2024Article
- Advances in genomics and genome editing for improving strawberry (Frontiers in genetics · 2024Review
- Foliar spraying of boron prolongs preservation period of strawberry fruits by altering boron form and boron distribution in cell.Frontiers in plant science · 2024Article
- Mechanism of abscisic acid in promoting softening of postharvest 'Docteur Jules Guyot' pear (Frontiers in plant science · 2024Article
- Deciphering the regulatory network of the NAC transcription factor FvRIF, a key regulator of strawberry (Fragaria vesca) fruit ripening.The Plant cell · 2023Article
- Comprehensive Analysis of the Pectate Lyase Gene Family and the Role ofInternational journal of molecular sciences · 2023Article
- Nano and Technological Frontiers as a Sustainable Platform for Postharvest Preservation of Berry Fruits.Foods (Basel, Switzerland) · 2023Review
- Genome-wide identification and expression analyses of the pectate lyase (PL) gene family in Fragaria vesca.BMC genomics · 2023Article
- Insights into cell wall changes during fruit softening from transgenic and naturally occurring mutants.Plant physiology · 2023Review
- Article
27 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Strawberry (Fragaria x ananassa, Duch., cv Chandler) is a soft fruit with a short postharvest life, mainly due to a rapid lost of firm texture. To control the strawberry fruit softening, we obtained transgenic plants that incorporate an antisense sequence of a strawberry pectate lyase gene under the control of the 35S promoter. Forty-one independent transgenic lines (Apel lines) were obtained, propagated in the greenhouse for agronomical analysis, and compared with control plants, non-transformed plants, and transgenic lines transformed with the pGUSINT plasmid. Total yield was significantly reduced in 33 of the 41 Apel lines. At the stage of full ripen, no differences in color, size, shape, and weight were observed between Apel and control fruit. However, in most of the Apel lines, ripened fruits were significantly firmer than controls. Six Apel lines were selected for further analysis. In all these lines, the pectate lyase gene expression in ripened fruit was 30% lower than in control, being totally suppressed in three of them. Cell wall material isolated from ripened Apel fruit showed a lower degree of in vitro swelling and a lower amount of ionically bound pectins than control fruit. An analysis of firmness at three different stages of fruit development (green, white, and red) showed that the highest reduction of softening in Apel fruit occurred during the transition from the white to the red stage. The postharvest softening of Apel fruit was also diminished. Our results indicate that pectate lyase gene is an excellent candidate for biotechnological improvement of fruit softening in strawberry.
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