ArticlePlant physiology2002
Temporal and spatial expression of a polygalacturonase during leaf and flower abscission in oilseed rape and Arabidopsis.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 111 citations in OpenAlex.
- Seed shattering habit in millets and the secrets of the abscission layer - a comprehensive review.Protoplasma · 2026Review
- Thidiazuron combined with cyclanilide modulates hormone pathways and ROS systems in cotton, increasing defoliation at low temperatures.Frontiers in plant science · 2024Article
- Genome-wide analysis of the mulberry (Frontiers in plant science · 2024Article
- Plant polygalacturonase structures specify enzyme dynamics and processivities to fine-tune cell wall pectins.The Plant cell · 2023Article
- Historical Selection, Adaptation Signatures, and Ambiguity of Introgressions in Wheat.International journal of molecular sciences · 2023Article
- Grain shattering by cell death and fracture in Eragrostis tef.Plant physiology · 2023Article
- Low Temperature Inhibits the Defoliation Efficiency of Thidiazuron in Cotton by Regulating Plant Hormone Synthesis and the Signaling Pathway.International journal of molecular sciences · 2022Article
- Integrated Transcriptome and Targeted Metabolite Analysis Reveal miRNA-mRNA Networks in Low-Light-Induced Lotus Flower Bud Abortion.International journal of molecular sciences · 2022Article
- A Non-Shedding FruitGenes · 2021Article
- Multi-scale comparative transcriptome analysis reveals key genes and metabolic reprogramming processes associated with oil palm fruit abscission.BMC plant biology · 2021Article
- Genome-wide identification and expression analysis of citrus fruitlet abscission-related polygalacturonase genes.3 Biotech · 2019Article
- Last-Generation Genome-Environment Associations Reveal the Genetic Basis of Heat Tolerance in Common Bean (Frontiers in genetics · 2019Article
- Comparative RNA-seq analysis of transcriptome dynamics during petal development in Rosa chinensis.Scientific reports · 2017Article
- Article
- Cell Wall Remodeling in Abscission Zone Cells during Ethylene-Promoted Fruit Abscission in Citrus.Frontiers in plant science · 2017Article
- Identification and Expression Analysis of Polygalacturonase Family Members during Peach Fruit Softening.International journal of molecular sciences · 2016Article
- Immunolocalization of pectic polysaccharides during abscission in pea seeds (Pisum sativum L.) and in abscission less def pea mutant seeds.BMC research notes · 2016Article
- Pectins, Endopolygalacturonases, and Bioenergy.Frontiers in plant science · 2016Review
- The Transcription Factor AtDOF4.7 Is Involved in Ethylene- and IDA-Mediated Organ Abscission in Arabidopsis.Frontiers in plant science · 2016Article
- Profiling gene expression in citrus fruit calyx abscission zone (AZ-C) treated with ethylene.Molecular genetics and genomics : MGG · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During leaf abscission in oilseed rape (Brassica napus), cell wall degradation is brought about by the action of several hydrolytic enzymes. One of these is thought to be polygalacturonase (PG). Degenerate primers were used to isolate a PG cDNA fragment by reverse transcriptase-polymerase chain reaction from RNA extracted from ethylene-promoted leaf abscission zones (AZs), and in turn a full-length clone (CAW471) from an oilseed rape AZ cDNA library. The highest homology of this cDNA (82%) was to an Arabidopsis sequence that was predicted to encode a PG protein. Analysis of expression revealed that CAW471 mRNA accumulated in the AZ of leaves and reached a peak 24 h after ethylene treatment. Ethylene-promoted leaf abscission in oilseed rape was not apparent until 42 h after exposure to the gas, reaching 50% at 48 h and 100% by 56 h. In floral organ abscission, expression of CAW471 correlated with cell separation. Genomic libraries from oilseed rape and Arabidopsis were screened with CAW471 and the respective genomic clones PGAZBRAN and PGAZAT isolated. Characterization of these PG genes revealed that they had substantial homology within both the coding regions and in the 5'-upstream sequences. Fusion of a 1,476-bp 5'-upstream sequence of PGAZAT to beta-glucuronidase or green fluorescent protein and transformation of Arabidopsis revealed that this fragment was sufficient to drive expression of these reporter genes in the AZs at the base of the anther filaments, petals, and sepals.
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