ArticlePlant physiology2002
Electrophysiological analysis of cloned cyclic nucleotide-gated ion channels.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed.
- Deciphering the Sodium Sensing Mechanisms in Glycophytes and Halophytes.Plant, cell & environment · 2025Review
- Lithium in plants.The New phytologist · 2025Review
- Structural basis for the activity regulation of Medicago calcium channel CNGC15.Cell discovery · 2025Article
- Genome-wide identification, characterization and expression analysis of CNGC Genes in moso bamboo (Phyllostachys edulis).BMC plant biology · 2025Article
- Genome-Wide Identification and Expression Analysis of the Cyclic Nucleotide-Gated Channel Gene Family inInternational journal of molecular sciences · 2024Article
- Rice NaFrontiers in plant science · 2024Article
- Ion Changes and Signaling under Salt Stress in Wheat and Other Important Crops.Plants (Basel, Switzerland) · 2023Review
- Genomic Insights into Adaptation to Karst Limestone and Incipient Speciation in East Asian Platycarya spp. (Juglandaceae).Molecular biology and evolution · 2023Article
- CaThe EMBO journal · 2022Review
- Plant salt response: Perception, signaling, and tolerance.Frontiers in plant science · 2022Review
- Moonlighting Proteins Shine New Light on Molecular Signaling Niches.International journal of molecular sciences · 2021Review
- The Complex Story of Plant Cyclic Nucleotide-Gated Channels.International journal of molecular sciences · 2021Review
- Functional Crypto-Adenylate Cyclases Operate in Complex Plant Proteins.Frontiers in plant science · 2021Article
- Extracellular Fragmented Self-DNA Is Involved in Plant Responses to Biotic Stress.Frontiers in plant science · 2021Article
- The CaFrontiers in plant science · 2021Article
- Cross Talk Between Cyclic Nucleotides and Calcium Signaling Pathways in Plants-Achievements and Prospects.Frontiers in plant science · 2021Review
- Plant Cyclic Nucleotide-Gated Channels: New Insights on Their Functions and Regulation.Plant physiology · 2020Article
- Ectopic overexpression of a cotton plastidial NaPlanta · 2020Article
- Calcium spikes, waves and oscillations in plant development and biotic interactions.Nature plants · 2020Review
- MgInternational journal of molecular sciences · 2020Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electrophysiological studies were conducted on the cloned plant cyclic nucleotide-gated ion channels AtCNGC2 and AtCNGC1 from Arabidopsis, and NtCBP4 from tobacco (Nicotiana tobacum). The nucleotide coding sequences for these proteins were expressed in Xenopus laevis oocytes or HEK 293 cells. Channel characteristics were evaluated using voltage clamp analysis of currents in the presence of cAMP. AtCNGC2 was demonstrated to conduct K(+) and other monovalent cations, but exclude Na(+); this conductivity profile is unique for any ion channel not possessing the amino acid sequence found in the selectivity filter of K(+)-selective ion channels. Application of cAMP evoked currents in membrane patches of oocytes injected with AtCNGC2 cRNA. Direct activation of the channel by cyclic nucleotide, demonstrated by application of cyclic nucleotide to patches of membranes expressing such channels, is a hallmark characteristic of this ion channel family. Voltage clamp studies (two-electrode configuration) demonstrated that AtCNGC1 and NtCBP4 are also cyclic nucleotide-gated channels. Addition of a lipophilic analog of cAMP to the perfusion bath of oocytes injected with NtCBP4 and AtCNGC1 cRNAs induced inward rectified, noninactivating K(+) currents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.