ArticleNucleic acids research2002
Functional and physical interactions between components of the Prp19p-associated complex.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 86 citations in OpenAlex.
- GPATCH11 ortholog Sap34 regulates pre-mRNA splicing by interacting with early spliceosomal complexes in Schizosaccharomyces pombe.Scientific reports · 2026Article
- The Prp19C/NTC subunit Syf2 and the Prp19C/NTC-associated protein Cwc15 function in TREX occupancy and transcription elongation.RNA (New York, N.Y.) · 2024Article
- A distinct complex of PRP19-related and trypanosomatid-specific proteins is required for pre-mRNA splicing in trypanosomes.Nucleic acids research · 2021Article
- Article
- Molecular Mechanisms of pre-mRNA Splicing through Structural Biology of the Spliceosome.Cold Spring Harbor perspectives in biology · 2019Review
- A new role for FBP21 as regulator of Brr2 helicase activity.Nucleic acids research · 2017Article
- A central role of Cwc25 in spliceosome dynamics during the catalytic phase of pre-mRNA splicing.RNA (New York, N.Y.) · 2017Article
- Role of Cwc24 in the First Catalytic Step of Splicing and Fidelity of 5' Splice Site Selection.Molecular and cellular biology · 2017Article
- Article
- CryoEM structures of two spliceosomal complexes: starter and dessert at the spliceosome feast.Current opinion in structural biology · 2016Review
- Small angle X-ray scattering studies of CTNNBL1 dimerization and CTNNBL1/CDC5L complex.Scientific reports · 2015Article
- Structural requirement of Ntc77 for spliceosome activation and first catalytic step.Nucleic acids research · 2014Article
- Structural and functional insights into the N-terminus of Schizosaccharomyces pombe Cdc5.Biochemistry · 2014Article
- Quantitative proteomic analysis reveals posttranslational responses to aneuploidy in yeast.eLife · 2014Article
- Molecular dissection of step 2 catalysis of yeast pre-mRNA splicing investigated in a purified system.RNA (New York, N.Y.) · 2013Article
- Malleable ribonucleoprotein machine: protein intrinsic disorder in the Saccharomyces cerevisiae spliceosome.PeerJ · 2013Article
- Functional roles of protein splicing factors.Bioscience reports · 2012Review
- Saccharomyces cerevisiae NineTeen complex (NTC)-associated factor Bud31/Ycr063w assembles on precatalytic spliceosomes and improves first and second step pre-mRNA splicing efficiency.The Journal of biological chemistry · 2012Article
- The spliceosome-activating complex: molecular mechanisms underlying the function of a pleiotropic regulator.Frontiers in plant science · 2012Article
- Disruption of murine mp29/Syf2/Ntc31 gene results in embryonic lethality with aberrant checkpoint response.PloS one · 2012Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Prp19p-associated complex is essential for the yeast pre-mRNA splicing reaction. The complex consists of at least eight protein components, but is not tightly associated with spliceosomal snRNAs. By a combination of genetic and biochemical methods we previously identified four components of this complex, Ntc25p, Ntc85p, Ntc30p and Ntc20p, all of them being novel splicing factors. We have now identified three other components of the complex, Ntc90p, Ntc77p and Ntc31p. These three proteins were also associated with the spliceosome during the splicing reaction in the same manner as Prp19p, concurrently with or immediately after dissociation of U4 snRNA. Two-hybrid analysis revealed that none of these proteins interacted with Prp19p or Ntc25p, but all interacted with Ntc85p. An interaction network between the identified components of the Prp19p-associated complex is demonstrated. Biochemical analysis revealed that Ntc90p, Ntc31p, Ntc30p and Ntc20p form a subcomplex, which, through interacting with Ntc85p and Ntc77p, can associate with Prp19p and Ntc25p to form the Prp19p-associated complex. Genetic analysis suggests that Ntc31p, Ntc30p and Ntc20p may play roles in modulating the function of Ntc90p.
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