ArticleNucleic acids research2002
Complete disproportionation of duplex poly(dT)*poly(dA) into triplex poly(dT)*poly(dA)*poly(dT) and poly(dA) by coralyne.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Coumarin 6H-fused fluorescent probe for highly sensitive detection of coralyne using oligonucleotide-modified silver nanoparticles.Analytical and bioanalytical chemistry · 2022Article
- A 'light-up' intercalator displacement assay for detection of triplex DNA stabilizers.Chemical communications (Cambridge, England) · 2020Article
- A tunable assay for modulators of genome-destabilizing DNA structures.Nucleic acids research · 2019Article
- Molecular rectifier composed of DNA with high rectification ratio enabled by intercalation.Nature chemistry · 2016Article
- Reprogramming the assembly of unmodified DNA with a small molecule.Nature chemistry · 2016Article
- Unexpected complex formation between coralyne and cyclic diadenosine monophosphate providing a simple fluorescent turn-on assay to detect this bacterial second messenger.Analytical chemistry · 2014Article
- Binding properties of Ru(II) polypyridyl complexes with poly(U)·poly(A)*poly(U) triplex: the ancillary ligand effect on third-strand stabilization.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2013Article
- Triplex inducer-directed self-assembly of single-walled carbon nanotubes: a triplex DNA-based approach for controlled manipulation of nanostructures.Nucleic acids research · 2011Article
- Calorimetric and spectroscopic studies of aminoglycoside binding to AT-rich DNA triple helices.Biochimie · 2010Article
- Intercalation as a means to suppress cyclization and promote polymerization of base-pairing oligonucleotides in a prebiotic world.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- New aspects of the interaction of the antibiotic coralyne with RNA: coralyne induces triple helix formation in poly(rA)*poly(rU).Nucleic acids research · 2010Article
- Polymorphic nucleic Acid binding of bioactive isoquinoline alkaloids and their role in cancer.Journal of nucleic acids · 2010Article
- Molecular dynamics simulations and coupled nucleotide substitution experiments indicate the nature of A{middle dot}A base pairing and a putative structure of the coralyne-induced homo-adenine duplex.Nucleic acids research · 2009Article
- Molecular recognition of poly(A) by small ligands: an alternative method of analysis reveals nanomolar, cooperative and shape-selective binding.Nucleic acids research · 2009Article
- Hydration changes in the association of Hoechst 33258 with DNA.Biochemistry · 2005Article
- Controlling nucleic acid secondary structure by intercalation: effects of DNA strand length on coralyne-driven duplex disproportionation.Nucleic acids research · 2003Article
- Spectroscopic studies of the multiple binding modes of a trimethine-bridged cyanine dye with DNA.Nucleic acids research · 2003Article
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2 authors.
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Abstract
Coralyne is a small crescent-shaped molecule known to intercalate duplex and triplex DNA. We report that coralyne can cause the complete and irreversible disproportionation of duplex poly(dT)*poly(dA). That is, coralyne causes the strands of duplex poly(dT)*poly(dA) to repartition into equal molar equivalents of triplex poly(dT)*poly(dA)*poly(dT) and poly(dA). Poly(dT)*poly(dA) will remain as a duplex for months after the addition of coralyne, if the sample is maintained at 4 degrees C. However, disproportionation readily occurs upon heating above 35 degrees C and is not reversed by subsequent cooling. A titration of poly(dT)*poly(dA) with coralyne reveals that disproportionation is favored by as little as one molar equivalent of coralyne per eight base pairs of initial duplex. We have also found that poly(dA) forms a self-structure in the presence of coralyne with a melting temperature of 47 degrees C, for the conditions of our study. This poly(dA) self-structure binds coralyne with an affinity that is comparable with that of triplex poly(dT)*poly(dA)*poly(dT). A Job plot analysis reveals that the maximum level of poly(dA) self-structure intercalation is 0.25 coralyne molecules per adenine base. This conforms to the nearest neighbor exclusion principle for a poly(dA) duplex structure with A*A base pairs. We propose that duplex disproportionation by coralyne is promoted by both the triplex and the poly(dA) self-structure having binding constants for coralyne that are greater than that of duplex poly(dT)*poly(dA).
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