ArticleNucleic acids research2002
The Schizosaccharomyces pombe mgU6-47 gene is required for 2'-O-methylation of U6 snRNA at A41.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 66 citations in OpenAlex.
- SNHG family lncRNAs promote Ewing sarcoma cell fitness through intronic snoRNA-guided rRNA modifications.NAR cancer · 2026Article
- The fission yeast methyl phosphate capping enzyme Bmc1 guides 2'-O-methylation of the U6 snRNA.Nucleic acids research · 2023Article
- The life of U6 small nuclear RNA, from cradle to grave.RNA (New York, N.Y.) · 2018Review
- Geneticin (G418) resistance and electroporation-mediated transformation ofBiotechnology, biotechnological equipment · 2015Article
- RTL-P: a sensitive approach for detecting sites of 2'-O-methylation in RNA molecules.Nucleic acids research · 2012Article
- MicroRNA expression profiling to identify and validate reference genes for relative quantification in colorectal cancer.BMC cancer · 2010Article
- SnoRNAs from the filamentous fungus Neurospora crassa: structural, functional and evolutionary insights.BMC genomics · 2009Article
- Identification of suitable endogenous control genes for microRNA gene expression analysis in human breast cancer.BMC molecular biology · 2008Article
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- snoSeeker: an advanced computational package for screening of guide and orphan snoRNA genes in the human genome.Nucleic acids research · 2006Article
- A novel experimental approach for systematic identification of box H/ACA snoRNAs from eukaryotes.Nucleic acids research · 2005Article
- Genome-wide analyses of two families of snoRNA genes from Drosophila melanogaster, demonstrating the extensive utilization of introns for coding of snoRNAs.RNA (New York, N.Y.) · 2005Article
- Cbf5p, the putative pseudouridine synthase of H/ACA-type snoRNPs, can form a complex with Gar1p and Nop10p in absence of Nhp2p and box H/ACA snoRNAs.RNA (New York, N.Y.) · 2004Article
- Identification of 20 microRNAs from Oryza sativa.Nucleic acids research · 2004Article
- The high diversity of snoRNAs in plants: identification and comparative study of 120 snoRNA genes from Oryza sativa.Nucleic acids research · 2003Article
- Computational comparison of two mouse draft genomes and the human golden path.Genome biology · 2003Article
- Plant snoRNA database.Nucleic acids research · 2003Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Through a computer search of DNA databases, we have identified the homologs of the mgU6-47 snoRNA gene from the yeast Schizosaccharomyces pombe, the fly Drosophila melanogaster and human. The three box C/D-containing snoRNA genes showed no significant similarity in their sequences except for an 11 nt long complementarity to U6 snRNA, suggesting that the mechanism of snoRNA guided snRNA methylation is conserved from mammals to yeast. The corresponding snoRNAs have been positively detected by reverse transcription and northern blotting. Taking advantage of the fission yeast system, we have disrupted the yeast mgU6-47 gene and demonstrated that it is absolutely required for site-specific 2'-O-methylation of U6 at position A41. No growth differences between mgU6-47 gene-disrupted and wild-type cells were observed, suggesting that the mgU6-47 gene, as for most rRNA methylation guides, is dispensable in yeast. Nevertheless, it was revealed by temperature shift assay that abolition of A41 methylation in yeast U6 snRNA might cause a small decrease in mRNA splicing efficiency. The timing of S.pombe U6 pre-RNA transport in the nucleus for splicing and methylation was also analyzed and is described.
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