ArticleGenetica2001
Improved fish lymphocyte culture for chromosome preparation.
Article in Genetica, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 4 citations in OpenAlex.
- GC and Repeats Profiling along Chromosomes-The Future of Fish Compositional Cytogenomics.Genes · 2020Article
- Cell culture-based karyotyping of orectolobiform sharks for chromosome-scale genome analysis.Communications biology · 2020Article
- Taxonomic Diversity Not Associated with Gross Karyotype Differentiation: The Case of Bighead Carps, GenusGenes · 2020Article
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- Molecular cytogenetic differentiation of paralogs of Hox paralogs in duplicated and re-diploidized genome of the North American paddlefish (Polyodon spathula).BMC genetics · 2017Article
- The second highest chromosome count among vertebrates is observed in cultured sturgeon and is associated with genome plasticity.Genetics, selection, evolution : GSE · 2016Article
- Reproductive isolation in a nascent species pair is associated with aneuploidy in hybrid offspring.Proceedings. Biological sciences · 2015Article
- Influence of β-glucan Leiber(®)Beta-S on selected innate immunity parameters of European eel (Anguilla anguilla) in an intensive farming system.Central-European journal of immunology · 2015Article
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- Developmental toxicity of PAH mixtures in fish early life stages. Part I: adverse effects in rainbow trout.Environmental science and pollution research international · 2014Article
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- Article
- Genome differentiation in a species pair of coregonine fishes: an extremely rapid speciation driven by stress-activated retrotransposons mediating extensive ribosomal DNA multiplications.BMC evolutionary biology · 2013Article
- Chromosomal study of a lamprey (Lampetra zanandreai Vladykov, 1955) (Petromyzonida: Petromyzontiformes): conventional and FISH analysis.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Article
- Integration of the genetic map and genome assembly of fugu facilitates insights into distinct features of genome evolution in teleosts and mammals.Genome biology and evolution · 2011Article
- Interchromosomal duplication of major histocompatibility complex class I regions in rainbow trout (Oncorhynchus mykiss), a species with a presumably recent tetraploid ancestry.Immunogenetics · 2005Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytogenetic methodology is still underdeveloped in fishes compared with mammals. Culture condition for fish lymphocytes was optimized to improve chromosome preparation using the rainbow trout (Oncorhynchus mykiss) as a model after changing the combination of parameters such as mitogens, incubation periods, media, cell components, and freshness of blood. The optimized culture condition included isolation of lymphocytes from fresh blood by a stirring method, their culture in medium 199 supplemented with 10% FBS, 18 microg/ml of phytohemagglutinin (PHA-W) and 100 microg/ml of lipopolysaccharide (LPS) as mitogens, and harvested at 6 days after culture. This condition provided a notably increased mitotic index (MI) of 4.3-10.0% in rainbow trout lymphocytes. In addition, the condition was highly reproducible as shown by the similar level of MI in cultured lymphocytes from 181 individuals without failure. Applicability of this method in a wide range of fish groups was also proven with Ml of 1.1-13.3% in cultured lymphocytes from other 16 freshwater species of Acipenseridae, Anguillidae, Solmonidae, Cyprinidae, and Centrarchidae, and five marine species of Sparidae, Kyphosidae, Paralichthyidae, and Scorpaenidae. Chromosome preparations of improved quality by the present method were successfully applied for the replication R-banding with incorporation of 5-bromo-2'-deoxyuridine and direct R-banding fluorescence in situ hybridization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.