ArticleMolecular and cellular biology2002
RNA sequence and base pairing effects on insertion editing in Trypanosoma brucei.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Article
- Comparative mitochondrial genome and transcriptome analyses reveal strain-specific features of RNA editing in Trypanosoma brucei.Nucleic acids research · 2025Article
- Structural basis of gRNA stabilization and mRNA recognition in trypanosomal RNA editing.Science (New York, N.Y.) · 2023Article
- Trypanosomatid, fluorescence-basedBio-protocol · 2021Article
- Analyzing editosome function in high-throughput.Nucleic acids research · 2020Article
- Lexis and Grammar of Mitochondrial RNA Processing in Trypanosomes.Trends in parasitology · 2020Review
- The RNA binding activity of the first identified trypanosome protein with Z-DNA-binding domains.Scientific reports · 2019Article
- U-Insertion/Deletion mRNA-Editing Holoenzyme: Definition in Sight.Trends in parasitology · 2016Review
- Review
- Identification of specific inhibitors for a trypanosomatid RNA editing reaction.RNA (New York, N.Y.) · 2010Article
- OB-fold domain of KREPA4 mediates high-affinity interaction with guide RNA and possesses annealing activity.RNA (New York, N.Y.) · 2010Article
- Article
- Mechanism of U insertion RNA editing in trypanosome mitochondria: the bimodal TUTase activity of the core complex.Journal of molecular biology · 2010Article
- Mechanism of U-insertion RNA editing in trypanosome mitochondria: characterization of RET2 functional domains by mutational analysis.Journal of molecular biology · 2010Article
- An electrochemiluminescent aptamer switch for a high-throughput assay of an RNA editing reaction.RNA (New York, N.Y.) · 2009Article
- Trypanosoma brucei ATPase subunit 6 mRNA bound to gA6-14 forms a conserved three-helical structure.RNA (New York, N.Y.) · 2008Article
- Determinants for association and guide RNA-directed endonuclease cleavage by purified RNA editing complexes from Trypanosoma brucei.Journal of molecular biology · 2008Article
- Terminal RNA uridylyltransferases of trypanosomes.Biochimica et biophysica acta · 2008Article
- Reconstitution of full-round uridine-deletion RNA editing with three recombinant proteins.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- RBP16 stimulates trypanosome RNA editing in vitro at an early step in the editing reaction.RNA (New York, N.Y.) · 2006Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
RNA editing inserts and deletes uridylates (U's) in kinetoplastid mitochondrial pre-mRNAs by a series of enzymatic steps. Small guide RNAs (gRNAs) specify the edited sequence. Editing, though sometimes extensive, is precise. The effects of mutating pre-mRNA and gRNA sequences in, around, and upstream of the editing site on the specificity and efficiency of in vitro insertion editing were examined. U's could be added opposite guiding pyrimidines, but guiding purines, particularly A's, were required for efficient ligation. A base pair between mRNA and gRNA immediately upstream of the editing site was not required for insertion editing, although it greatly enhanced its efficiency and accuracy. In addition, a gRNA/mRNA duplex upstream of the editing site enhanced insertion editing when it was close to the editing site, but prevented cleavage, and hence editing, when immediately adjacent to the editing site. Thus, several aspects of mRNA-gRNA interaction, as well as gRNA base pairing with added U's, optimize editing efficiency, although they are not required for insertion editing.
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Registered trials
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