ArticleMolecular pathology : MP2002
Detection of genes expressed in primary colon cancers by in situ hybridisation: overexpression of RACK 1.
Article in Molecular pathology : MP, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
- The Apoptotic, Angiogenic and Cell Proliferation Genes CD63, S100A6 e GNB2L1 are Altered in Patients with Endometriosis.Revista brasileira de ginecologia e obstetricia : revista da Federacao Brasileira das Sociedades de Ginecologia e Obstetricia · 2018Article
- High expression of RACK1 is associated with poor prognosis in patients with pancreatic ductal adenocarcinoma.Oncology letters · 2018Article
- Extracellular matrix gene expression profiling using microfluidics for colorectal carcinoma stratification.Biomicrofluidics · 2016Article
- Microfluidic droplet-based PCR instrumentation for high-throughput gene expression profiling and biomarker discovery.Biomolecular detection and quantification · 2015Article
- Overexpressed RACK1 is positively correlated with malignant degree of human colorectal carcinoma.Molecular biology reports · 2014Article
- Identification and characteristics of a novel gene, EJO1, in the Chinese mitten crab (Eriocheir japonica sinensis) ovary.Molecular biology reports · 2010Article
- Transcription analysis in the MeLiM swine model identifies RACK1 as a potential marker of malignancy for human melanocytic proliferation.Molecular cancer · 2008Article
- Proof concept for clinical justification of network mapping for personalized cancer therapeutics.Cancer gene therapy · 2007Article
- Rewired ERK-JNK signaling pathways in melanoma.Cancer cell · 2007Article
- RACK1 mediates activation of JNK by protein kinase C [corrected].Molecular cell · 2005Article
- Construction and selection of subtracted cDNA library of mouse hepatocarcinoma cell lines with different lymphatic metastasis potential.World journal of gastroenterology · 2004Article
- Tumour biological aspects of CD24, a mucin-like adhesion molecule.Journal of molecular histology · 2004Review
- Differential gene-expression profiles associated with gastric adenoma.British journal of cancer · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsThe isolation of various genes that are expressed in a region specific manner is considered useful for research in molecular pathology. In situ hybridisation (ISH) was used in a screening procedure to isolate these genes efficiently, using colon cancer as a model.
methodsSuppression subtractive hybridisation (SSH) between colon cancer tissue samples and corresponding non-cancerous tissues was performed. Genes showing high expression in the cancers were selected using macro-DNA array analysis. As a final screening procedure, conventional ISH was performed to isolate genes expressed specifically in colon cancers.
resultsSixty nine clones were selected by SSH and macro-DNA array analyses. These clones were then analysed by ISH to examine their expression patterns. ISH screening revealed that all the clones screened showed more intense signals in colon cancers than in non-cancerous tissues. Among them, RACK 1, which is a protein kinase C receptor and a homologue of the G protein beta subunit, was expressed intensely in colon cancer cells. RACK 1 expression was evaluated in multiple samples by ISH, and the results confirmed that RACK 1 was universally overexpressed in cells of all 11 colon cancers examined.
conclusionsMany genes, including RACK 1, expressed in colon cancer cells can be isolated efficiently by this method, and their precise expression pattern can be evaluated. These results indicate that ISH is an excellent technique for systemic screening of genes expressed in a region specific manner.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.