ArticleJournal of virology2002
Identification of a type 1 peroxisomal targeting signal in a viral protein and demonstration of its targeting to the organelle.
Article in Journal of virology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 50 citations in OpenAlex.
- Review
- Hepatitis C virus alters the morphology and function of peroxisomes.Frontiers in microbiology · 2023Article
- Peroxisomes and Viruses: Overview on Current Knowledge and Experimental Approaches.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Re-Examining Rotavirus Innate Immune Evasion: Potential Applications of the Reverse Genetics System.mBio · 2022Review
- Morphology Remodeling and Selective Autophagy of Intracellular Organelles during Viral Infections.International journal of molecular sciences · 2020Review
- The C Terminus of Rotavirus VP4 Protein Contains an Actin Binding Domain Which Requires Cooperation with the Coiled-Coil Domain for Actin Remodeling.Journal of virology · 2019Article
- Localization of Protein Kinase NDR2 to Peroxisomes and Its Role in Ciliogenesis.The Journal of biological chemistry · 2017Article
- A paradox of transcriptional and functional innate interferon responses of human intestinal enteroids to enteric virus infection.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Flavivirus Infection Impairs Peroxisome Biogenesis and Early Antiviral Signaling.Journal of virology · 2015Article
- Peroxisome-mitochondria interplay and disease.Journal of inherited metabolic disease · 2015Review
- Article
- A tuberous sclerosis complex signalling node at the peroxisome regulates mTORC1 and autophagy in response to ROS.Nature cell biology · 2013Article
- Peroxisomes and the antiviral responses of mammalian cells.Sub-cellular biochemistry · 2013Review
- Integration of peroxisomes into an endomembrane system that governs cellular aging.Frontiers in physiology · 2012Article
- The proteoglycan bamacan is a host cellular ligand of vaccinia virus neurovirulence factor N1L.Journal of neurovirology · 2009Article
- Predicted mouse peroxisome-targeted proteins and their actual subcellular locations.BMC bioinformatics · 2008Article
- Application of bioinformatics-coupled experimental analysis reveals a new transport-competent nuclear localization signal in the nucleoprotein of influenza A virus strain.BMC cell biology · 2008Article
- Defective rotavirus particle assembly in lovastatin-treated MA104 cells.Archives of virology · 2008Article
- Rotavirus spike protein VP4 binds to and remodels actin bundles of the epithelial brush border into actin bodies.Journal of virology · 2006Article
- Rotavirus assembly: an alternative model that utilizes an atypical trafficking pathway.Current topics in microbiology and immunology · 2006Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Peroxisomes are unimembrane, respiratory organelles of the cell. Transport of cellular proteins to the peroxisomal matrix requires a type 1 peroxisomal targeting signal (PTS1) which essentially constitutes a tripeptide from the consensus sequence S/T/A/G/C/N-K/R/H-L/I/V/M/A/F/Y. Although PTS-containing proteins have been identified in eukaryotes, prokaryotes, and parasites, viral proteins with such signals have not been identified so far. We report here the first instance of a virus, the rotavirus, which causes infantile diarrhea worldwide, containing a functional C-terminal PTS1 in one of its proteins (VP4). Analysis of 153 rotavirus VP4-deduced amino acid sequences identified five groups of conserved C-terminal PTS1 tripeptide sequences (SKL, CKL, GKL, CRL, and CRI), of which CRL is represented in approximately 62% of the sequences. Infection of cells by a CRL-containing representative rotavirus (SA11 strain) and confocal immunofluorescence analysis revealed colocalization of VP4 with peroxisomal markers and morphological changes of peroxisomes. Further, transient cellular expression of green fluorescent protein (GFP)-fused VP4CRL resulted in transport of VP4 to peroxisomes, whereas the chimera lacking the PTS1 signal, GFP-VP4DeltaCRL, resulted in diffuse cytoplasmic staining, suggesting a CRL-dependent targeting of the protein. The present study therefore demonstrates hitherto unreported organelle involvement, specifically of the peroxisomes, in rotaviral infections as demonstrated by using the SA11 strain of rotavirus and opens a new line of investigation toward understanding viral pathogenesis and disease mechanisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.