ArticleBritish journal of pharmacology2002
Inhibition of adenylyl cyclase by neuronal P2Y receptors.
Article in British journal of pharmacology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 40 citations in OpenAlex.
- Caffeinated Beverage Intake, Dyspnea With Ticagrelor, and Cardiovascular Outcomes: Insights From the PEGASUS-TIMI 54 Trial.Journal of the American Heart Association · 2020Trial
- Role and recent progress of P2Y12 receptor in cancer development.Purinergic signalling · 2025Review
- Long Non-coding RNA Uc.48+ Small Interfering RNA Alleviates Neuroinflammatory Hyperalgesia in Gp120-Treated Rats via the P2Y12 Receptor.Frontiers in neuroscience · 2021Article
- Spinal αBritish journal of pharmacology · 2019Review
- Activation of microglial P2Y12 receptor is required for outward potassium currents in response to neuronal injury.Neuroscience · 2016Article
- P2Y12 receptor expression is a critical determinant of functional responsiveness to ATX's MORFO domain.Purinergic signalling · 2012Article
- P2Y1 receptors mediate an activation of neuronal calcium-dependent K+ channels.The Journal of physiology · 2010Article
- Autoregulation in PC12 cells via P2Y receptors: Evidence for non-exocytotic nucleotide release from neuroendocrine cells.Purinergic signalling · 2007Article
- International Union of Pharmacology LVIII: update on the P2Y G protein-coupled nucleotide receptors: from molecular mechanisms and pathophysiology to therapy.Pharmacological reviews · 2006Review
- Synthesis of pyridoxal phosphate derivatives with antagonist activity at the P2Y13 receptor.Biochemical pharmacology · 2005Article
- Cerebellar astrocytes co-express several ADP receptors. Presence of functional P2Y(13)-like receptors.Purinergic signalling · 2005Article
- Activity-dependent autocrine-paracrine activation of neuronal P2Y receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- P2Y-receptors stimulating the proliferation of human mesangial cells through the MAPK42/44 pathway.British journal of pharmacology · 2003Article
- P2Y receptor regulation of cultured rat cerebral cortical cells: calcium responses and mRNA expression in neurons and glia.British journal of pharmacology · 2003Article
- Attenuation of the P2Y receptor-mediated control of neuronal Ca2+ channels in PC12 cells by antithrombotic drugs.British journal of pharmacology · 2003Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
P2Y receptors inhibiting adenylyl cyclase have been found in blood platelets, glioma cells, and endothelial cells. In platelets and glioma cells, these receptors were identified as P2Y(12). Here, we have used PC12 cells to search for adenylyl cyclase inhibiting P2Y receptors in a neuronal cellular environment. ADP and ATP (0.1 - 100 microM) left basal cyclic AMP accumulation unaltered, but reduced cyclic AMP synthesis stimulated by activation of endogenous A(2A) or recombinant beta(2) receptors. Forskolin-dependent cyclic AMP production was reduced by <or=1 microM and enhanced by 10 - 100 microM ADP; this latter effect was turned into an inhibition when A(2A) receptors were blocked. The nucleotide inhibition of cyclic AMP synthesis was not altered when P2X receptors were blocked, but abolished by pertussis toxin. The rank order of agonist potencies for the reduction of cyclic AMP was (IC(50) values): 2-methylthio-ADP (0.12 nM)=2-methylthio-ATP (0.13 nM)>ADPbetaS (71 nM)>ATP (164 nM)=ADP (244 nM). The inhibition by ADP was not antagonized by suramin, pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid, or adenosine-3'-phosphate-5'-phosphate, but attenuated by reactive blue 2, ATP(alpha)S, and 2-methylthio-AMP. RT - PCR demonstrated the expression of P2Y(2), P2Y(4), P2Y(6), and P2Y(12), but not P2Y(1), receptors in PC12 cells. In Northern blots, only P2Y(2) and P2Y(12) were detectable. Differentiation with NGF did not alter these hybridization signals and left the nucleotide inhibition of adenylyl cyclase unchanged. We conclude that P2Y(12) receptors are expressed in neuronal cells and inhibit adenylyl cyclase activity.
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