ArticleBritish journal of pharmacology2002
Spontaneous phasic activity of the pig urinary bladder smooth muscle: characteristics and sensitivity to potassium channel modulators.
Article in British journal of pharmacology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 99 citations in OpenAlex.
- Cells and ionic conductances contributing to spontaneous activity in bladder and urethral smooth muscle.The Journal of physiology · 2026Review
- Urothelium-derived prostanoids enhance contractility of urinary bladder smooth muscle and stimulate bladder afferent nerve activity in the mouse.American journal of physiology. Regulatory, integrative and comparative physiology · 2024Article
- Role of PTHrP in attenuating transient pressure rises and associated afferent nerve activity of the rat bladder.Pflugers Archiv : European journal of physiology · 2022Article
- Reviving Cav1.2 as an attractive drug target to treat bladder dysfunction.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Review
- Drugs Currently Undergoing Preclinical or Clinical Trials for the Treatment of Overactive Bladder: A Review.Current therapeutic research, clinical and experimental · 2022Review
- Partial recovery of voiding function in female mice following repeated psychological stress exposure.PloS one · 2022Article
- TRPM4 channel inhibitors 9-phenanthrol and glibenclamide differentially decrease guinea pig detrusor smooth muscle whole-cell cation currents and phasic contractions.American journal of physiology. Cell physiology · 2020Article
- Effect of high-fat diet-induced obesity on the small-conductance CaInternational urology and nephrology · 2019Article
- Modulation of lower urinary tract smooth muscle contraction and relaxation by the urothelium.Naunyn-Schmiedeberg's archives of pharmacology · 2018Review
- Effect of pregnancy on cooling tone and rhythmic contractions of the rat urinary bladder.International urology and nephrology · 2018Article
- Regulation of Spontaneous Contractions in Intact Rat Bladder Strips and the Effects of Hydrogen Peroxide.BioMed research international · 2018Article
- Low amplitude rhythmic contraction frequency in human detrusor strips correlates with phasic intravesical pressure waves.World journal of urology · 2017Article
- Acetylcholinesterase Inhibitors and Drugs Acting on Muscarinic Receptors- Potential Crosstalk of Cholinergic Mechanisms During Pharmacological Treatment.Current neuropharmacology · 2017Review
- Original Research: Combined model of bladder detrusor smooth muscle and interstitial cells.Experimental biology and medicine (Maywood, N.J.) · 2016Article
- Transient contractions of urinary bladder smooth muscle are drivers of afferent nerve activity during filling.The Journal of general physiology · 2016Article
- Regulation of urinary bladder function by protein kinase C in physiology and pathophysiology.BMC urology · 2015Review
- NS19504: a novel BK channel activator with relaxing effect on bladder smooth muscle spontaneous phasic contractions.The Journal of pharmacology and experimental therapeutics · 2014Article
- BK channel activators and their therapeutic perspectives.Frontiers in physiology · 2014Review
- NS309 decreases rat detrusor smooth muscle membrane potential and phasic contractions by activating SK3 channels.British journal of pharmacology · 2013Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A hallmark for unstable bladder contractions is hyperexcitability and changes in the nature of spontaneous phasic activity of the bladder smooth muscle. In this study, we have characterized the spontaneous activity of the urinary bladder smooth muscle from the pig, a widely used model for studying human bladder function. Our studies demonstrate that phasic activity of the pig detrusor is myogenic and is influenced by the presence of urothelium. Denuded strips exhibit robust spontaneous activity measured as mean area under the contraction curve (AUC=188.9+/-15.63 mNs) compared to intact strips (AUC=7.3+/-1.94 mNs). Spontaneous phasic activity, particularly the amplitude, is dependent on both calcium entry through voltage-dependent calcium channels and release from ryanodine receptors as shown by inhibition of spontaneous activity by nifedipine and ryanodine respectively. Inhibition of BK(Ca) channels by iberiotoxin (100 nM) resulted in an increase in contraction amplitude (89.1+/-20.4%) and frequency (92.5+/-31.0%). The SK(Ca) channel blocker apamin (100 nM) also increased contraction amplitude (69.1+/-24.3%) and frequency (53.5+/-13.6%) demonstrating that these mechanisms are critical to the regulation of phasic spontaneous activity. Inhibition of K(ATP) channels by glyburide (10 microM) did not significantly alter myogenic contractions (AUC=18.5+/-12.3%). However, K(ATP) channel openers (KCOs) showed an exquisite sensitivity for suppression of spontaneous myogenic activity. KCOs were generally 15 fold more potent in suppressing spontaneous activity compared to contractions evoked by electrical field-stimulation. These studies suggest that potassium channel modulation, particularly K(ATP) channels, may offer a unique mechanism for controlling spontaneous myogenic activity especially those associated with the hyperexcitability occurring in unstable bladders.
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