ArticleWorld journal of gastroenterology2002
Upregulation of vascular endothelial growth factor by hydrogen peroxide in human colon cancer.
Article in World journal of gastroenterology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 34 citations in OpenAlex.
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- Overexpression of catalase delays G0/G1- to S-phase transition during cell cycle progression in mouse aortic endothelial cells.Free radical biology & medicine · 2009Article
- Enzyme-digested Fucoidan Extracts Derived from Seaweed Mozuku of Cladosiphon novae-caledoniae kylin Inhibit Invasion and Angiogenesis of Tumor Cells.Cytotechnology · 2005Article
- Nuclear factor-kappaB p65 (RelA) transcription factor is constitutively activated in human colorectal carcinoma tissue.World journal of gastroenterology · 2004Article
- Expression of NF-kappaB and human telomerase reverse transcriptase in gastric cancer and precancerous lesions.World journal of gastroenterology · 2004Article
- Inhibitory effects of docetaxel on expression of VEGF, bFGF and MMPs of LS174T cell.World journal of gastroenterology · 2003Article
- Variability of cell proliferation in the proximal and distal colon of normal rats and rats with dimethylhydrazine induced carcinogenesis.World journal of gastroenterology · 2002Article
- Arterial chemotherapy of 5-fluorouracil and mitomycin C in the treatment of liver metastases of colorectal cancer.World journal of gastroenterology · 2002Article
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5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
aimTo evaluate the effect of reactive oxygen species such as hydrogen peroxide on the progression of human colon cancer.
methodsHuman colon carcinoma cell lines, LS174T and HCT8, were treated respectively with 10(-5), 10(-7) or 10(-9) mol x L(-1) hydrogen peroxide for 24h,and co-cultured with human endothelial cell line ECV-304. The migration of ECV-304 induced by cancer cells was calculated and the expression level of vascular endothelial growth factor in cancer cells was determined by RT-PCR analysis and ELISA. Dactinomycin of 1.5mg x L(-1) which could block transcription of cancer cells was applied to observing the effects of H(2)O(2) on transcriptional activity and the relative half-life of VEGF mRNA. Finally,to evaluate the effect of H2O2 on NF-kappaB activity in colon cancer cells, NF-kappaB in cytoplasm and nucleus of the cells were detected with FITC-tagged antibody and its presence in the nucleus(Fn) vs cytoplasm(Fc) was monitored by measuring the green fluorescence integrated over the nucleus by laser scanning cytometry(LSC).
resultsExogenouse hydrogen peroxide of low concentration increased the migration of endothelial cell induced by colon cancer cells. When cancer cells were treated with 10(-5) mol x L(-1) H2O2, the migration number of endothelial cells induced by LS174T cells was 203+/-70 and the number induced by HCT8 cells was 145+/-65. The two values were significantly higher than those treated with other concentrations of H2O2 (P<0.01). The expression of vascular endothelial growth factor in cancer cells, which could be blocked by dactinomycin, were increased to a certain degree, while the relative half-life of VEGF mRNA was not prolonged after treatment with hydrogen peroxide. The activity of NF-kappaB in colon cells rose after the cells were exposed to hydrogen peroxide for 24h. The Fn values in HCT8 cells were 91+/-13 (0 mol x L(-1) H2O2) and 149+/-40(10(-5) mol x L(-1) H2O2)(P<0.05), in LS174T cells were 127+/-35(0 mol x L(-1) H2O2) and 192+/-11(10(-5)mol x L(-1) H2O2) (P<0.05). It is similar to the case of VEGF expression in cancer cells.
conclusionHydrogen peroxide increases vascular endothelial growth factor expression in colon cancer cells, and it is likely that reactive oxygen species such as hydrogen peroxide facilitates the development of colon cancer.
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