ArticleWorld journal of gastroenterology2002
Direct effect of croton oil on intestinal epithelial cells and colonic smooth muscle cells.
Article in World journal of gastroenterology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Purgative Effect, Acute Toxicity, and Quantification of Phorbol-12-Myristate-13-Acetate and Crotonic Acid inInternational journal of molecular sciences · 2025Article
- Alleviation of Collagen-Induced Arthritis by Crotonoside through Modulation of Dendritic Cell Differentiation and Activation.Plants (Basel, Switzerland) · 2020Article
- Article
- Phorbol 12-Myristate 13-Acetate-Induced Changes in Chicken Enterocytes.Proteomics insights · 2019Article
- Assessment of acute, 14-day, and 13-week repeated oral dose toxicity of Tiglium seed extract in rats.BMC complementary and alternative medicine · 2018Article
- Article
- Crotonis Fructus and Its Constituent, Croton Oil, Stimulate Lipolysis in OP9 Adipocytes.Evidence-based complementary and alternative medicine : eCAM · 2014Article
- PMA induces vaccine adjuvant activity by the modulation of TLR signaling pathway.Mediators of inflammation · 2014Article
- Detoxification of Croton tiglium L. seeds by Ayurvedic process of Śodhana.Ancient science of life · 2014Article
- Antinociceptive and Smooth Muscle Relaxant Activity of Croton tiglium L Seed: An In-vitro and In-vivo Study.Iranian journal of pharmaceutical research : IJPR · 2012Article
- Analysis of the Potential Topical Anti-Inflammatory Activity of Averrhoa carambola L. in Mice.Evidence-based complementary and alternative medicine : eCAM · 2011Article
- Comparative evaluation of immune response after laparoscopical and open total mesorectal excisions with anal sphincter preservation in patients with rectal cancer.World journal of gastroenterology · 2003Article
- Effects of neurotrophins on gastrointestinal myoelectric activities of rats.World journal of gastroenterology · 2003Article
- Peroxisome proliferator-activated receptor gamma ligands inhibit cell growth and induce apoptosis in human liver cancer BEL-7402 cells.World journal of gastroenterology · 2003Article
- PPARgamma pathway activation results in apoptosis and COX-2 inhibition in HepG2 cells.World journal of gastroenterology · 2003Article
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9 authors.
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No grant is acknowledged in the PubMed record.
Abstract
aimTo investigate the direct effect of croton oil (CO) on human intestinal epithelial cell (HIEC) and guinea pig colonic smooth muscle cells in vitro.
methodsGrowth curves of HIEC were drawn by MTT colorimetry. The dynamics of cell proliferation was analyzed with flow cytometry, and morphological changes were observed under light and electron microscopy after long-term (6 weeks) treatment with CO. Expression of cyclooxygenase-2 (COX-2) mRNA was detected by dot blot in HIEC treated with CO. Genes related to CO were screened by DD-PCR, and the direct effect of CO on the contractility of isolated guinea pig colonic smooth muscle cells was observed.
resultsHigh concentration (20-40 mg x L(-1)) CO inhibited cell growth significantly (1, 3, 5, 7d OD sequence: (20 mg x L(-1)) 0.040+/-0.003, 0.081+/-0.012, 0.147+/-0.022,0.024+/-0.016; (40 mg x L(-1)) 0.033+/-0.044, 0.056+/-0.012, 0.104+/-0.010, 0.189+/-0.006; OD control 0.031+/-0.008, 0.096+/-0.012, 0.173+/-0.009, 0.300+/-0.016, P<0.01), which appeared to be related directly to the dosage. Compared with the control, the fraction number of cells in G1 phase decreased from 0.60 to 0.58, while that in S phase increased from 0.30 to 0.34 and DNA index also increased after 6 weeks of treatment with CO (the dosage was increased gradually from 4 to 40 mg x L(-1)). Light microscopic observation revealed that cells had karyomegaly, less plasma and karyoplasm lopsidedness. Electron microscopy also showed an increase in cell proliferation and in the quantity of abnormal nuclei with pathologic mitosis. Expression of COX-2 mRNA decreased significantly in HIEC treated with CO. Thirteen differential cDNA fragments were cloned from HIEC treated with CO, one of which was 100 percent homologous with human mitochondrial cytochrome C oxidase subunit II. The length of isolated guinea pig colonic smooth muscle cells was significantly shortened after treatment with CO (P<0.05).
conclusionAt a high CO concentration (>20 mg x L(-1)), cell growth and proliferation are inhibited in a dosage-dependent manner. Increase in cell proliferation and in malignant conversion of the cellular phenotype is observed in cells cultured chronically with CO. COX-2 mRNA expression decreases significantly, while human mitochondrial cytochrome C oxidase subunit IImRNA expression increases significantly in HIEC treated with CO. CO also has a direct effect on the contractility of Guinea pig colonic smooth muscle cells.
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