ArticleWorld journal of gastroenterology2002
VEGF165 antisense RNA suppresses oncogenic properties of human esophageal squamous cell carcinoma.
Article in World journal of gastroenterology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Vascular endothelial growth factor antisense oligonucleotides inhibit leptomeningeal metastasis in vivo.Medical oncology (Northwood, London, England) · 2011Article
- Inhibitory effect of antisense vascular endothelial growth factor RNA on the profile of hepatocellular carcinoma cell line in vitro and in vivo.World journal of gastroenterology · 2006Article
- Antiangiogenic gene therapy of cancer: recent developments.Journal of translational medicine · 2004Article
- Synthesis of ribozyme against vascular endothelial growth factor165 and its biological activity in vitro.World journal of gastroenterology · 2004Article
- Vascular endothelial growth factor antisense oligodeoxynucleotides with lipiodol in arterial embolization of liver cancer in rats.World journal of gastroenterology · 2004Article
- Relationship between inducible nitric oxide synthase expression and angiogenesis in primary gallbladder carcinoma tissue.World journal of gastroenterology · 2004Article
- Effects of depression on parameters of cell-mediated immunity in patients with digestive tract cancers.World journal of gastroenterology · 2004Article
- Inhibition of adenovirus-mediated p27kip1 gene on growth of esophageal carcinoma cell strain.World journal of gastroenterology · 2003Article
- Separation and identification of differentially expressed nuclear matrix proteins between human esophageal immortalized and carcinomatous cell lines.World journal of gastroenterology · 2003Article
- Inhibitory effects of docetaxel on expression of VEGF, bFGF and MMPs of LS174T cell.World journal of gastroenterology · 2003Article
- HLA-DRB1 allele polymorphisms in genetic susceptibility to esophageal carcinoma.World journal of gastroenterology · 2003Article
- Expression of e-cadherin and beta-catenin in human esophageal squamous cell carcinoma: relationships with prognosis.World journal of gastroenterology · 2003Article
- Preoperative TN staging of esophageal cancer: comparison of miniprobe ultrasonography, spiral CT and MRI.World journal of gastroenterology · 2003Article
- Expression of vascular endothelial growth factor and its receptors KDR and Flt-1 in gastric cancer cells.World journal of gastroenterology · 2002Article
- Identification of differentially expressed proteins between human esophageal immortalized and carcinomatous cell lines by two-dimensional electrophoresis and MALDI-TOF-mass spectrometry.World journal of gastroenterology · 2002Article
- Subcellular daunorubicin distribution and its relation to multidrug resistance phenotype in drug-resistant cell line SMMC-7721/R.World journal of gastroenterology · 2002Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo investigate the effect of antisense RNA to vascular endothelial growth factor165 (VEGF165) on human esophageal squamous cell carcinoma cell line EC109 and the feasibility of gene therapy for esophageal carcinoma.
methodsBy using subclone technique, the full length of VEGF165 amino acid cDNA, which was cut from pGEM-3Zf+,was cloned inversely into the eukaryotic expression vector pCEP4. The recombinant plasmid pCEP-AVEGF165 was transfected into EC109 cell with lipofectamine. After a stable transfection, dot blot, enzyme-linked immunosorbent assay (ELISA),laser confocal imaging system analysis, transmission electron microscopy and flow cytometry were performed to determine the biological characteristics of EC109 cell line before and after transfection in vitro and whether there was a reversion in the tumorigenic properties of the EC109 cell in vitro.
resultsThe eukaryotic expression vector pCEP-AVEGF165 was successfully constructed and transfected into EC109 cells. The expression of VEGF165 was significantly decreased in the transfected cells while the biological characteristics of the cells were not influenced by the expression of antisense gene. The tumorigenic and angiogenic capabilities were greatly reduced in nude mice, as demonstrated by reduced tumor end volume (820+/-112.5)mm(3) vs (7930+/-1035)mm(3) and (7850+/-950)mm(3), P<0.01) and microvessel density (average number: (8.5+/-1.2)mm(-2) vs (44.3+/-9.4)mm(-2) and (46.4+/-12.6)mm(-2), P<0.01) in comparison between experimental groups empty vector transfected group and control group.
conclusionThe angiogenesis and tumorigenicity of human esophageal squamous cell carcinoma were effectively inhibited by VEGF165 antisense RNA. Antisense RNA to VEGF165 can potentially be used as an adjuvant therapy for solid tumors.
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