ArticleWorld journal of gastroenterology2002
Nitric oxide and calcium ions in apoptotic esophageal carcinoma cells induced by arsenite.
Article in World journal of gastroenterology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 30 citations in OpenAlex.
- Unraveling the Role of α2δ-1 in Cerebral Hemorrhage: Calcium Overload, Endoplasmic Reticulum Stress, and Microglial Apoptosis.Brain and behavior · 2025Article
- Conjunctival Fluid Secretion Impairment via CaCC-CFTR Dysfunction Is the Key Mechanism in Environmental Dry Eye.International journal of molecular sciences · 2022Article
- Pannexin-1 Contributes to the Apoptosis of Spinal Neurocytes in Spinal Cord Injury.Frontiers in physiology · 2021Article
- Synergistic Carcinogenesis of HPV18 and MNNG in Het-1A Cells through p62-KEAP1-NRF2 and PI3K/AKT/mTOR Pathway.Oxidative medicine and cellular longevity · 2020Article
- Intracellular calcium disturbances induced by arsenic and its methylated derivatives in relation to genomic damage and apoptosis induction.Environmental health perspectives · 2005Review
- Effects of depression on parameters of cell-mediated immunity in patients with digestive tract cancers.World journal of gastroenterology · 2004Article
- Protective role of metallothionein (I/II) against pathological damage and apoptosis induced by dimethylarsinic acid.World journal of gastroenterology · 2004Article
- Expression properties of recombinant pEgr-P16 plasmid in esophageal squamous cell carcinoma induced by ionizing irradiation.World journal of gastroenterology · 2003Article
- Separation and identification of differentially expressed nuclear matrix proteins between human esophageal immortalized and carcinomatous cell lines.World journal of gastroenterology · 2003Article
- Effects of hydroxyapatite nanoparticles on proliferation and apoptosis of human hepatoma BEL-7402 cells.World journal of gastroenterology · 2003Article
- HLA-DRB1 allele polymorphisms in genetic susceptibility to esophageal carcinoma.World journal of gastroenterology · 2003Article
- Preoperative TN staging of esophageal cancer: comparison of miniprobe ultrasonography, spiral CT and MRI.World journal of gastroenterology · 2003Article
- Identification of differentially expressed proteins between human esophageal immortalized and carcinomatous cell lines by two-dimensional electrophoresis and MALDI-TOF-mass spectrometry.World journal of gastroenterology · 2002Article
- Subcellular daunorubicin distribution and its relation to multidrug resistance phenotype in drug-resistant cell line SMMC-7721/R.World journal of gastroenterology · 2002Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo Quantitatively analyze the nitric oxide (NO) and Ca2+ in apoptosis of esophageal carcinoma cells induced by arsenic trioxide (As2O3).
methodsThe cell line SHEEC1, a malignant esophageal epithelial cell induced by HPV in synergy with TPA in our laboratory, was cultured in a serum-free medium and treated with As2O3. Before and after administration of As2O3, NO production in cultured medium was detected quantitatively using the Griess Colorimetric method. Intracellular Ca2+ was labeled by using the fluorescent dye Fluo3-AM and detected under confocal laser scanning microscope (CLSM), which was able to acquire data in real-time enabling Ca2+ dynamics of individual cells in vitro. The apoptotic cells were examined under electron microscopy.
resultsIntracellular concentration of Ca2+ increased from 1.00 units to 1.09-1.38 units of fluorescent intensity at As2O3 treatment and NO products subsequently released from As2O3-treated cells increased from 0.98-1.00 x10(-2)micromol x L(-1) up to 1.48-1.52 x10(-2)micromol x L(-1) and maintained in a high level continuously. Finally apoptosis of cells occurred,chromatin being agglutinated, cells shrunk,nuclei became round and mitochondria swelled.
conclusionCa2+ and NO increased with cell damage and apoptosis in cells treated by As2O3. The Ca2+ is an initial messenger to the apoptotic pathway. To investigate Ca2+ and NO will be a new direction for studying the apoptotic signaling messenger of the esophageal carcinoma cells induced by As2O3.
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