ArticleWorld journal of gastroenterology2002
Morphological and functional changes of mitochondria in apoptotic esophageal carcinoma cells induced by arsenic trioxide.
Article in World journal of gastroenterology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 43 citations in OpenAlex.
- An ingenious non-spherical mesoporous silica nanoparticle cargo with curcumin induces mitochondria-mediated apoptosis in breast cancer (MCF-7) cells.Oncotarget · 2019Article
- Evidence of hormesis on human neuronal SK-N-BE cells treated with sodium arsenate: impact at the mitochondrial level.Environmental science and pollution research international · 2016Article
- Enhancement of arsenic trioxide-mediated changes in human induced pluripotent stem cells (IPS).International journal of environmental research and public health · 2014Article
- Arsenic trioxide enhances the cytotoxic effect of cisplatin in head and neck squamous cell carcinoma cell lines.Oncology letters · 2012Article
- Low-intensity ultrasound-induced cellular destruction and autophagy of nasopharyngeal carcinoma cells.Experimental and therapeutic medicine · 2011Article
- Mitochondrial microsatellite instability in gastric cancer and its precancerous lesions.World journal of gastroenterology · 2004Article
- Nuclear and mitochondrial DNA microsatellite instability in hepatocellular carcinoma in Chinese.World journal of gastroenterology · 2004Article
- Protective role of metallothionein (I/II) against pathological damage and apoptosis induced by dimethylarsinic acid.World journal of gastroenterology · 2004Article
- Separation and identification of differentially expressed nuclear matrix proteins between human esophageal immortalized and carcinomatous cell lines.World journal of gastroenterology · 2003Article
- Variations of mitochondrial D-loop region plus downstream gene 1 2S rRNA-tRNA(phe) and gastric carcinomas.World journal of gastroenterology · 2003Article
- Effects of hydroxyapatite nanoparticles on proliferation and apoptosis of human hepatoma BEL-7402 cells.World journal of gastroenterology · 2003Article
- Synergistic effects of nimesulide and 5-fluorouracil on tumor growth and apoptosis in the implanted hepatoma in mice.World journal of gastroenterology · 2003Article
- HLA-DRB1 allele polymorphisms in genetic susceptibility to esophageal carcinoma.World journal of gastroenterology · 2003Article
- Preoperative TN staging of esophageal cancer: comparison of miniprobe ultrasonography, spiral CT and MRI.World journal of gastroenterology · 2003Article
- Identification of differentially expressed proteins between human esophageal immortalized and carcinomatous cell lines by two-dimensional electrophoresis and MALDI-TOF-mass spectrometry.World journal of gastroenterology · 2002Article
- Changes of NF-kB, p53, Bcl-2 and caspase in apoptosis induced by JTE-522 in human gastric adenocarcinoma cell line AGS cells: role of reactive oxygen species.World journal of gastroenterology · 2002Article
- JTE-522-induced apoptosis in human gastric adenocarcinoma [correction of adenocarcinoma] cell line AGS cells by caspase activation accompanying cytochrome C release, membrane translocation of Bax and loss of mitochondrial membrane potential.World journal of gastroenterology · 2002Article
- Nitric oxide and calcium ions in apoptotic esophageal carcinoma cells induced by arsenite.World journal of gastroenterology · 2002Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo demonstrate that mitochondrial morphological and functional changes are an important intermediate link in the course of apoptosis in esophageal carcinoma cells induced by As2O3.
methodsThe esophageal carcinoma cell line SHEEC1, established in our laboratory, was cultured in 199 growth medium, supplemented with 100mL x L(-1) calf serum and 3 mol x L(-1)As2O3 (the same below). After 2, 4, 6, 12, 24 h of drug adding, the SHEEC1 cells were collected for light-and electron-microscopic examination. The mitochondria were labeled by Rhodamine fluorescence probe and the fluorescence intensity of the mitochondria was measured by flow cytometer and cytofluorimetric analysis. Further,the mitochondrial transmembrane potential (MTP, psim) change was also calculated.
resultsThe mitochondrial morphological change after adding As2O3 could be divided into three stages. In the early-stage (2-6h) after adding As2O3, an adaptive proliferation of mitochondria appeared; in the mid-stage (6-12 h) a degenerative change was observed; and in the late-stage (12-24 h) the mitochondria swelled with outer membrane broken down and then cells death with apoptotic changes of nucleus. The functional change of the mitochondria indicated by fluorescent intensity, which reflected the MTP status of mitochondria, was in accordance with morphological change of the mitochondria. The fluorescent intensity increased at early-stage, declined in mid-stage and decreased to the lowest in the late-stage. 24 h after As2O3 adding, the cell nucleus showed typical apoptotic changes.
conclusionUnder the inducement of As2O3, the early apoptotic changes of SHEEC1 cells were the apparent morphological and functional changes of mitochondria, afterwards the nucleus changes followed. It is considered that changes of mitochondria are an important intermediate link in the course of apoptosis of esophageal carcinoma cells induced by As2O3.
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