Evidence map›Paper›PMID 11832339›Full record

ArticleAmerican journal of physiology. Cell physiology2002

Adenoviral gene transfer of PDGF downregulates gas gene product PDGFalphaR and prolongs ERK and Akt/PKB activation.

Qi-Ping Chen, William V Giannobile

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of physiology. Cell physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
0.7field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 29 citations in OpenAlex.

  1. Article
  2. Review
  3. Gene therapy with growth factors for periodontal tissue engineering--a review.Medicina oral, patologia oral y cirugia bucal · 2012
    Review
  4. Review
  5. Article
  6. Article
  7. Craniofacial tissue engineering by stem cells.Journal of dental research · 2006
    Review
  8. Review
  9. Gene therapeutics for periodontal regenerative medicine.Dental clinics of North America · 2006
    Review
  10. Engineering of tooth-supporting structures by delivery of PDGF gene therapy vectors.Molecular therapy : the journal of the American Society of Gene Therapy · 2004
    Article
  11. Article
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Qi-Ping ChenCenter for Biorestoration of Oral Health, Department of Periodontics, University of Michigan, 1011 N. University Ave., Ann Arbor, MI 48109-1078, USA.
William V Giannobile
University of Michigan–Ann Arbor · US

Funding

GENE THERAPY FOR RECONSTRUCTING PERIODONTAL TISSUESR01DE013397 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI GIANNOBILE, WILLIAM V · 2000 to 2014
$4.0M
PDGF-MEDIATED GENE TRANSFER &PERIODONTAL WOUND HEALINGR29DE011960 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI GIANNOBILE, WILLIAM V · 1997 to 2001
$239k
NIDCR NIH HHS DE 11960NIDCR NIH HHS DE 13397NIDCR NIH HHS R01 DE013397NIDCR NIH HHS R29 DE011960
6 · The paper itself

Abstract

The delivery of platelet-derived growth factor (PDGF) for tissue engineering of skin and periodontal wounds has become an active area of interest. However, little is known regarding the extended effects of PDGF on cell signaling via gene therapy and how such an approach facilitates the exiting of cells from growth arrest and entry to competence required for cell cycling. We show in vitro expression and secretion of PDGF-AA by recombinant adenovirus encoding the PDGF-A gene (Ad-PDGF-A). The bioactive PDGF-AA protein released induces sustained downregulation of PDGFalphaR that is encoded by a growth arrest-specific (gas) gene. Ad-PDGF-A induces sustained phosphorylation of PDGFalphaR as well as prolonged phosphorylation of downstream extracellular signal-regulated kinase 1/2 and Akt signaling pathways. Furthermore, the phosphorylation of PDGFalphaR is abolished by cotransducing cells with adenovirus encoding a dominant negative mutant of the PDGF-A gene that disrupts PDGF bioactivity. These findings demonstrate the prolonged effects of adenoviral delivery of PDGF and aid in the better understanding of sustained PDGF signaling.

Indexed as

Protein Serine-Threonine KinasesAdenoviridaeAnimalsCell CycleCells, CulturedCulture Media, Serum-FreeDown-RegulationFibroblastsGene Transfer TechniquesHumansMitogen-Activated Protein KinasesPhosphorylationPlatelet-Derived Growth FactorProtein IsoformsProto-Oncogene ProteinsProto-Oncogene Proteins c-aktAKT1 protein, humanAkt1 protein, ratCulture Media, Serum-FreeMitogen-Activated Protein KinasesPlatelet-Derived Growth Factorplatelet-derived growth factor AProtein IsoformsProtein Serine-Threonine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktReceptor, Platelet-Derived Growth Factor alpha

Identifiers

PMID11832339
PMCPMC2579768
OpenAlexW2115245951

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.