ArticleProceedings of the National Academy of Sciences of the United States of America2002
The analysis of 100 genes supports the grouping of three highly divergent amoebae: Dictyostelium, Entamoeba, and Mastigamoeba.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers.
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101 citing papers in PubMed, 376 citations in OpenAlex.
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- DNA Barcodes Combined with Multilocus Data of Representative Taxa Can Generate Reliable Higher-Level Phylogenies.Systematic biology · 2022Article
- The Mastigamoeba balamuthi Genome and the Nature of the Free-Living Ancestor of Entamoeba.Molecular biology and evolution · 2021Article
- Intrinsic laws of k-mer spectra of genome sequences and evolution mechanism of genomes.BMC evolutionary biology · 2020Article
- Releasing uncurated datasets is essential for reproducible phylogenomics.Nature ecology & evolution · 2020Article
- Single cell ecology.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Molecular characterization of Phytophthora palmivora responsible for bud rot disease of oil palm in Colombia.World journal of microbiology & biotechnology · 2019Article
- The first set of universal nuclear protein-coding loci markers for avian phylogenetic and population genetic studies.Scientific reports · 2018Article
- The Nothoaspis amazoniensis Complete Mitogenome: A Comparative and Phylogenetic Analysis.Veterinary sciences · 2018Article
- NommPred: Prediction of Mitochondrial and Mitochondrion-Related Organelle Proteins of Nonmodel Organisms.Evolutionary bioinformatics online · 2018Article
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- Evolution of Cell-Autonomous Effector Mechanisms in Macrophages versus Non-Immune Cells.Microbiology spectrum · 2016Review
- Phylogenetic Resolution of Deep Eukaryotic and Fungal Relationships Using Highly Conserved Low-Copy Nuclear Genes.Genome biology and evolution · 2016Article
- Phylogeny Reconstruction with Alignment-Free Method That Corrects for Horizontal Gene Transfer.PLoS computational biology · 2016Article
- Entamoeba mitosomes play an important role in encystation by association with cholesteryl sulfate synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Classification and expression analyses of homeobox genes from Dictyostelium discoideum.Journal of biosciences · 2015Article
41 more citing papers are in PubMed but not listed here.
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11 authors at 1 institution in 2 countries.
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Abstract
The phylogenetic relationships of amoebae are poorly resolved. To address this difficult question, we have sequenced 1,280 expressed sequence tags from Mastigamoeba balamuthi and assembled a large data set containing 123 genes for representatives of three phenotypically highly divergent major amoeboid lineages: Pelobionta, Entamoebidae, and Mycetozoa. Phylogenetic reconstruction was performed on approximately 25,000 aa positions for 30 species by using maximum-likelihood approaches. All well-established eukaryotic groups were recovered with high statistical support, validating our approach. Interestingly, the three amoeboid lineages strongly clustered together in agreement with the Conosa hypothesis [as defined by T. Cavalier-Smith (1998) Biol. Rev. Cambridge Philos. Soc. 73, 203-266]. Two amitochondriate amoebae, the free-living Mastigamoeba and the human parasite Entamoeba, formed a significant sister group to the exclusion of the mycetozoan Dictyostelium. This result suggested that a part of the reductive process in the evolution of Entamoeba (e.g., loss of typical mitochondria) occurred in its free-living ancestors. Applying this inexpensive expressed sequence tag approach to many other lineages will surely improve our understanding of eukaryotic evolution.
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