ArticleThe Journal of cell biology2002
The yeast protein kinase Mps1p is required for assembly of the integral spindle pole body component Spc42p.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 73 citations in OpenAlex.
- Phosphate-binding pocket on cyclin B governs CDK substrate phosphorylation and mitotic timing.Nature communications · 2025Article
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- Phosphotyrosine picked threonine kinase stimulates proliferation of human osteosarcoma cellsArchives of medical science : AMS · 2022Article
- Enzymatic Noncovalent Synthesis.Chemical reviews · 2020Review
- Structure and function of Spc42 coiled-coils in yeast centrosome assembly and duplication.Molecular biology of the cell · 2019Article
- Novel phosphorylation states of the yeast spindle pole body.Biology open · 2018Article
- Key phosphorylation events in Spc29 and Spc42 guide multiple steps of yeast centrosome duplication.Molecular biology of the cell · 2018Article
- Review
- Article
- Interaction of CK1δ with γTuSC ensures proper microtubule assembly and spindle positioning.Molecular biology of the cell · 2015Article
- Review
- Mad2, Bub3, and Mps1 regulate chromosome segregation and mitotic synchrony in Giardia intestinalis, a binucleate protist lacking an anaphase-promoting complex.Molecular biology of the cell · 2014Article
- Mps1 and Ipl1/Aurora B act sequentially to correctly orient chromosomes on the meiotic spindle of budding yeast.Science (New York, N.Y.) · 2013Article
- Article
- Mitotic spindle form and function.Genetics · 2012Article
- The MPS1 family of protein kinases.Annual review of biochemistry · 2012Review
- Centriole assembly and the role of Mps1: defensible or dispensable?Cell division · 2011Article
- The G1 phase Cdks regulate the centrosome cycle and mediate oncogene-dependent centrosome amplification.Cell division · 2011Article
- Antizyme restrains centrosome amplification by regulating the accumulation of Mps1 at centrosomes.Molecular biology of the cell · 2010Article
- N-terminal regions of Mps1 kinase determine functional bifurcation.The Journal of cell biology · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Saccharomyces cerevisiae MPS1 encodes an essential protein kinase that has roles in spindle pole body (SPB) duplication and the spindle checkpoint. Previously characterized MPS1 mutants fail in both functions, leading to aberrant DNA segregation with lethal consequences. Here, we report the identification of a unique conditional allele, mps1-8, that is defective in SPB duplication but not the spindle checkpoint. The mutations in mps1-8 are in the noncatalytic region of MPS1, and analysis of the mutant protein indicates that Mps1-8p has wild-type kinase activity in vitro. A screen for dosage suppressors of the mps1-8 conditional growth phenotype identified the gene encoding the integral SPB component SPC42. Additional analysis revealed that mps1-8 exhibits synthetic growth defects when combined with certain mutant alleles of SPC42. An epitope-tagged version of Mps1p (Mps1p-myc) localizes to SPBs and kinetochores by immunofluorescence microscopy and immuno-EM analysis. This is consistent with the physical interaction we detect between Mps1p and Spc42p by coimmunoprecipitation. Spc42p is a substrate for Mps1p phosphorylation in vitro, and Spc42p phosphorylation is dependent on Mps1p in vivo. Finally, Spc42p assembly is abnormal in a mps1-1 mutant strain. We conclude that Mps1p regulates assembly of the integral SPB component Spc42p during SPB duplication.
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Registered trials
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