ArticleThe Journal of cell biology2002
Disassembly of interchromatin granule clusters alters the coordination of transcription and pre-mRNA splicing.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
What it found
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Who cites it
62 citing papers in PubMed, 153 citations in OpenAlex.
- Coordination of nuclear RNA processing by speckle-localized kinase TAOK2.Genes & development · 2026Article
- Phosphorylation of a nuclear condensate regulates cohesion and mRNA retention.Nature communications · 2025Article
- Nuclear speckle biology: At the cross-roads of discovery and functional analysis.Current opinion in cell biology · 2024Review
- Phosphorylation of the nuclear poly(A) binding protein (PABPN1) during mitosis protects mRNA from hyperadenylation and maintains transcriptome dynamics.Nucleic acids research · 2024Article
- Microscopic Analysis of Nuclear Speckles in a Viviparous Reptile.International journal of molecular sciences · 2024Article
- Article
- CDK11 requires a critical activator SAP30BP to regulate pre-mRNA splicing.The EMBO journal · 2023Article
- Pharmacological perturbation of the phase-separating protein SMNDC1.Nature communications · 2023Article
- CLK1 reorganizes the splicing factor U1-70K for early spliceosomal protein assembly.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Article
- Cdc-Like Kinases (CLKs): Biology, Chemical Probes, and Therapeutic Potential.International journal of molecular sciences · 2020Review
- Nuclear export of chimeric mRNAs depends on an lncRNA-triggered autoregulatory loop in blood malignancies.Cell death & disease · 2020Article
- Availability of splicing factors in the nucleoplasm can regulate the release of mRNA from the gene after transcription.PLoS genetics · 2019Article
- Chromosome Territorial Organization Drives Efficient Protein Complex Formation: A Hypothesis.The Yale journal of biology and medicine · 2019Review
- Disordered protein interactions for an ordered cellular transition: Cdc2-like kinase 1 is transported to the nucleus via its Ser-Arg protein substrate.The Journal of biological chemistry · 2019Article
- Molecular interactions connecting the function of the serine-arginine-rich protein SRSF1 to protein phosphatase 1.The Journal of biological chemistry · 2018Article
- Mobilization of a splicing factor through a nuclear kinase-kinase complex.The Biochemical journal · 2018Article
- Fanconi anemia FANCD2 and FANCI proteins regulate the nuclear dynamics of splicing factors.The Journal of cell biology · 2017Article
- Redirecting SR Protein Nuclear Trafficking through an Allosteric Platform.Journal of molecular biology · 2017Article
- Persistent nuclear actin filaments inhibit transcription by RNA polymerase II.Journal of cell science · 2016Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
To examine the involvement of interchromatin granule clusters (IGCs) in transcription and pre-mRNA splicing in mammalian cell nuclei, the serine-arginine (SR) protein kinase cdc2-like kinase (Clk)/STY was used as a tool to manipulate IGC integrity in vivo. Both immunofluorescence and transmission electron microscopy analyses of cells overexpressing Clk/STY indicate that IGC components are completely redistributed to a diffuse nuclear localization, leaving no residual structure. Conversely, overexpression of a catalytically inactive mutant, Clk/STY(K190R), causes retention of hypophosphorylated SR proteins in nuclear speckles. Our data suggest that the protein-protein interactions responsible for the clustering of interchromatin granules are disrupted when SR proteins are hyperphosphorylated and stabilized when SR proteins are hypophosphorylated. Interestingly, cells without intact IGCs continue to synthesize nascent transcripts. However, both the accumulation of splicing factors at sites of pre-mRNA synthesis as well as pre-mRNA splicing are dramatically reduced, demonstrating that IGC disassembly perturbs coordination between transcription and pre-mRNA splicing in mammalian cell nuclei.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.