ArticleThe Journal of molecular diagnostics : JMD2002
Core biopsies can be used to distinguish differences in expression profiling by cDNA microarrays.
Article in The Journal of molecular diagnostics : JMD, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 44 citations in OpenAlex.
- Khasianine Affects the Expression of Sugar-Sensitive Proteins in Pancreatic Cancer Cells, Which Are Altered in Data from the Rat Model and Patients.ACS pharmacology & translational science · 2023Article
- High-throughput molecular analysis from leftover of fine needle aspiration cytology of mammographically detected breast cancer.Translational oncology · 2012Article
- High-throughput molecular and histopathologic profiling of tumor tissue in a novel transplantable model of murine neuroblastoma: new tools for pediatric drug discovery.Cancer investigation · 2012Article
- A comparison of RNA amplification techniques at sub-nanogram input concentration.BMC genomics · 2009Article
- Microarray gene expression profiling using core biopsies of renal neoplasia.American journal of translational research · 2009Article
- Diagnostic and prognostic sarcoma signatures.Molecular diagnosis & therapy · 2008Review
- BRCA1 and BRCA2: chemosensitivity, treatment outcomes and prognosis.Familial cancer · 2006Review
- Construction of tissue microarrays from prostate needle biopsy specimens.British journal of cancer · 2005Article
- Database of mRNA gene expression profiles of multiple human organs.Genome research · 2005Article
- Prediction of clinical outcome using gene expression profiling and artificial neural networks for patients with neuroblastoma.Cancer research · 2004Article
- Role of gene expression arrays in sarcomas.Current oncology reports · 2004Review
- Microarray analysis in clinical oncology: pre-clinical optimization using needle core biopsies from xenograft tumors.BMC cancer · 2004Article
- Utility of linearly amplified RNA for RT-PCR detection of chromosomal translocations: validation using the t(2;5)(p23;q35) NPM-ALK chromosomal translocation.The Journal of molecular diagnostics : JMD · 2004Article
- A comparative analysis of data generated using two different target preparation methods for hybridization to high-density oligonucleotide microarrays.BMC genomics · 2004Article
- Breast cancer classification and prognosis based on gene expression profiles from a population-based study.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- The c-myc x E2F-1/p21 interactive gene expression index augments cytomorphologic diagnosis of lung cancer in fine-needle aspirate specimens.The Journal of molecular diagnostics : JMD · 2003Article
- Effects of mRNA amplification on gene expression ratios in cDNA experiments estimated by analysis of variance.BMC genomics · 2003Article
- Gene expression profiles derived from fine needle aspiration correlate with response to systemic chemotherapy in breast cancer.Breast cancer research : BCR · 2002Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The primary focus of this work was to determine the feasibility of obtaining representative expression array profiles from clinical core biopsies. For this purpose we performed six 16-gauge needle core biopsies and an excision biopsy on each of two different human xenografts, one from an Ewing's sarcoma cell line and the second from neuroblastoma cell line grown in Beige-Scid mice. Three of the six core biopsies were processed separately and the remaining three were pooled and processed together. As the initial RNA material isolated from the core biopsies was not sufficient for microarray analysis, an amplification procedure using a modified Eberwine protocol was performed, and the amplified products applied onto a 6000-feature human cDNA microarray. Comparisons of the array results from core biopsies (amplified RNA) and surgical specimens (non-amplified RNA) showed maintenance of the expression profile as assessed by hierarchical clustering. Gene expression profiles obtained from microarray analysis clearly differentiated the Ewing's sarcoma from the neuroblastoma with both core and excisional biopsies as starting material. Pooling the core biopsies did not improve the concordance with excisional biopsies. In conclusion, our results suggest that core biopsies can be used as a suitable and reliable material for the determination of tumor genetic profiles.
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