ArticleThe Journal of physiology2002
Ca2+ transport properties and determinants of anomalous mole fraction effects of single voltage-gated Ca2+ channels in hair cells from bullfrog saccule.
Article in The Journal of physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 41 citations in OpenAlex.
- Cation Permeability of Voltage-Gated Hair Cell CaInternational journal of molecular sciences · 2022Article
- Recent advances in cochlear hair cell nanophysiology: subcellular compartmentalization of electrical signaling in compact sensory cells.Faculty reviews · 2020Review
- Voltage-Gated Calcium Channels: Key Players in Sensory Coding in the Retina and the Inner Ear.Physiological reviews · 2018Review
- Voltage control of Ca²⁺ permeation through N-type calcium (Ca(V)2.2) channels.The Journal of general physiology · 2014Article
- Genetic, cellular, and functional evidence for Ca2+ inflow through Cav1.2 and Cav1.3 channels in murine spiral ganglion neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Posthearing Ca(2+) currents and their roles in shaping the different modes of firing of spiral ganglion neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Release from the cone ribbon synapse under bright light conditions can be controlled by the opening of only a few Ca(2+) channels.Journal of neurophysiology · 2011Article
- Selectivity sequences in a model calcium channel: role of electrostatic field strength.European biophysics journal : EBJ · 2011Article
- Sieving experiments and pore diameter: it's not a simple relationship.European biophysics journal : EBJ · 2010Article
- Release and elementary mechanisms of nitric oxide in hair cells.Journal of neurophysiology · 2010Article
- Identification of changes in the functional profile of the cardiac ryanodine receptor caused by the coupled gating phenomenon.The Journal of membrane biology · 2010Article
- Vesicle pool size at the salamander cone ribbon synapse.Journal of neurophysiology · 2010Article
- Synaptic ribbon enables temporal precision of hair cell afferent synapse by increasing the number of readily releasable vesicles: a modeling study.Journal of neurophysiology · 2008Article
- Effects of strontium on the permeation and gating phenotype of calcium channels in hair cells.Journal of neurophysiology · 2008Article
- Molecular identity and functional properties of a novel T-type Ca2+ channel cloned from the sensory epithelia of the mouse inner ear.Journal of neurophysiology · 2008Article
- Energetics of divalent selectivity in a calcium channel: the ryanodine receptor case study.Biophysical journal · 2008Article
- Development and regeneration of hair cells share common functional features.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Ca2+ current of frog vestibular hair cells is modulated by intracellular ATP but not by long-lasting depolarisation.European biophysics journal : EBJ · 2007Article
- Creation of a biological pacemaker by gene- or cell-based approaches.Medical & biological engineering & computing · 2007Review
- Hair cell ribbon synapses.Cell and tissue research · 2006Review
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
We studied the permeation properties of two distinct single voltage-gated Ca2+ channels in bullfrog saccular hair cells to assess the roles of the channels as physiological Ca2+ transporters and multi-ion pores. By varying the permeant ions (Ba2+, Ca2+) and concentrations (2-70 mM), we estimated the affinity constant (K(D)) of the two channels as follows (mM): L-type channel, K(D,Ba) = 7.4 +/- 1.0, K(D,Ca) = 7.1 +/- 2.2 (n = 7); non-L-type channel, K(D,Ba) = 5.3 +/- 3.2, K(D,Ca) = 2.0 +/- 1.0 (n = 8). Using ionic concentrations close to physiological conditions (2 mM Ca2+ and 1.0 mM Mg2+), the conductance of the L-type channel was approximately 2 pS. We determined the mechanisms by which ions traverse the pore of these single Ca2+ channels, using mixtures of Ba2+ and Ca2+ at total concentrations above (70 mM) or close to (5 mM) the K(D) of the channels. We found evidence for an anomalous mole fraction effect (AMFE) only when the total divalent ion concentration was 5 mM, consistent with a multi-ion pore. We show that AMFE arises from the boundaries between the pore and bulk solution in the atria of the channel, which is derived from the presence of depletion zones that become apparent at low divalent cation concentrations. The present findings provide an explanation as to why previous whole-cell Ca2+ currents that were recorded in quasi-physiological Ca2+ concentrations (approximately 2-5 mM) showed clear AMFE, whereas single Ca2+ channel currents that were recorded routinely at high Ca2+ concentrations (20-110 mM) did not.
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