ArticleJournal of clinical microbiology2002
Evaluation of a rapid PCR-based epidemiological typing method for routine studies of Mycobacterium tuberculosis.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Relapse or Re-Infection, the Situation of Recurrent Tuberculosis in Eastern China.Frontiers in cellular and infection microbiology · 2021Article
- Characterization of mutations in the rpoB gene conferring rifampicin resistance in Mycobacterium tuberculosis complex isolated from lymph nodes of slaughtered cattle from South Africa.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Clonality and genetic profiles of drug-resistant Mycobacterium tuberculosis in the Eastern Cape Province, South Africa.MicrobiologyOpen · 2019Article
- Molecular typing of Mycobacterium tuberculosis complex isolated from pulmonary tuberculosis patients in central Ethiopia.BMC infectious diseases · 2017Article
- Current methods in the molecular typing of Mycobacterium tuberculosis and other mycobacteria.BioMed research international · 2014Review
- Spoligotypes of Mycobacterium tuberculosis from different Provinces of China.Journal of clinical microbiology · 2010Article
- Evaluation of low-colony-number counts of Mycobacterium tuberculosis on solid media as a microbiological marker of cross-contamination.Journal of clinical microbiology · 2009Article
- Colorimetric detection of multidrug-resistant or extensively drug-resistant tuberculosis by use of malachite green indicator dye.Journal of clinical microbiology · 2008Article
- Differentiation of tuberculosis strains in a population with mainly Beijing-family strains.Emerging infectious diseases · 2006Article
- Progression toward an improved DNA amplification-based typing technique in the study of Mycobacterium tuberculosis epidemiology.Journal of clinical microbiology · 2006Article
- Rifampicin resistance in tuberculosis outbreak, London, England.Emerging infectious diseases · 2005Article
- Detection of mutations associated with isoniazid and rifampin resistance in Mycobacterium tuberculosis isolates from Samara Region, Russian Federation.Journal of clinical microbiology · 2004Article
- Article
- Statistical approaches for estimating actinobacterial diversity in marine sediments.Applied and environmental microbiology · 2003Article
- Evaluation and utilization as a public health tool of a national molecular epidemiological tuberculosis outbreak database within the United Kingdom from 1997 to 2001.Journal of clinical microbiology · 2003Article
- Rifampin- and multidrug-resistant tuberculosis in Russian civilians and prison inmates: dominance of the beijing strain family.Emerging infectious diseases · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Restriction fragment length polymorphism (RFLP) based on the insertion sequence IS6110 is used to investigate episodes of suspected transmission of infection of tuberculosis but usually takes a number of weeks from receipt of request to obtain a result. Often investigations would benefit from a more rapid method, possibly one containing an amplification step. The method employed uses a simple DNA extraction followed by a PCR step involving a single primer. Restriction enzyme analysis was performed when the patterns obtained from the PCR products were indistinguishable, especially when only single similar-size bands were obtained. The isolates used were strains of Mycobacterium tuberculosis submitted for epidemiological investigations as part of (i) possible contact-outbreak (22 episodes involving between 2 and 20 patients), (ii) possible incidents of laboratory cross-contamination (21 episodes), and (iii) possible change in drug resistance pattern or a case of reinfection (1 patient). The PCR products giving similar patterns were then subjected to restriction enzyme analysis. In conclusion it has been shown that this method is rapid, with results within 1 to 2 days of the request being received; is reproducible; and gives the same results as does RFLP. The restriction enzyme analysis stage has improved the efficiency of the technique.
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