ArticleJournal of clinical microbiology2002
Development and evaluation of real-time PCR-based fluorescence assays for detection of Chlamydia pneumoniae.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A meta-analysis of antibiotic use for the secondary prevention of cardiovascular diseases.The Canadian journal of cardiology · 2008Pooled it
- Effect of clarithromycin treatment on Chlamydia pneumoniae in vascular tissue of patients with coronary artery disease: a randomized, double-blind, placebo-controlled trial.Journal of clinical microbiology · 2005Trial
- Epidemiological changes inEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2025Article
- From coughs to complications: the story ofJournal of medical microbiology · 2025Review
- ERA-CRISPR/Cas12a-based, fast and specific diagnostic detection forFrontiers in cellular and infection microbiology · 2024Article
- Genetic diversity of Rickettsia africae isolates from Amblyomma hebraeum and blood from cattle in the Eastern Cape province of South Africa.Experimental & applied acarology · 2020Article
- AssayingMolecules (Basel, Switzerland) · 2020Article
- Factors associated with fatal cases of acute respiratory infection (ARI) among hospitalized patients in Guatemala.BMC public health · 2019Article
- Genomic factors related to tissue tropism in Chlamydia pneumoniae infection.BMC genomics · 2015Article
- Development and validation of a real-time PCR for Chlamydia suis diagnosis in swine and humans.PloS one · 2014Article
- What is the role of newer molecular tests in the management of CAP?Infectious disease clinics of North America · 2013Review
- Article
- The urgent need for robust coral disease diagnostics.PLoS pathogens · 2011Review
- Verification of the ProPneumo-1 assay for the simultaneous detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae in clinical respiratory specimens.Annals of clinical microbiology and antimicrobials · 2009Article
- Article
- Genotyping of Chlamydophila psittaci by real-time PCR and high-resolution melt analysis.Journal of clinical microbiology · 2009Article
- Development and evaluation of a novel multiplex PCR technology for molecular differential detection of bacterial respiratory disease pathogens.Journal of clinical microbiology · 2008Article
- Simultaneous detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae by use of molecular beacons in a duplex real-time PCR.Journal of clinical microbiology · 2008Article
- Currently used nucleic acid amplification tests for the detection of viruses and atypicals in acute respiratory infections.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2007Review
- Detection of amyloid beta aggregates in the brain of BALB/c mice after Chlamydia pneumoniae infection.Acta neuropathologica · 2007Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chlamydia pneumoniae is an important respiratory pathogen recently associated with atherosclerosis and several other chronic diseases. Detection of C. pneumoniae is inconsistent, and standardized PCR assays are needed. Two real-time PCR assays specific for C. pneumoniae were developed by using the fluorescent dye-labeled TaqMan probe-based system. Oligonucleotide primers and probes were designed to target two variable domains of the ompA gene, VD2 and VD4. The limit of detection for each of the two PCR assays was 0.001 inclusion-forming unit. Thirty-nine C. pneumoniae isolates obtained from widely distributed geographical areas were amplified by the VD2 and VD4 assays, producing the expected 108- and 125-bp amplification products, respectively. None of the C. trachomatis serovars, C. psittaci strains, other organisms, or human DNAs tested were amplified. The amplification results of the newly developed assays were compared to the results of culturing and two nested PCR assays, targeting the 16S rRNA and ompA genes. The assays were compared by testing C. pneumoniae purified elementary bodies, animal tissues, 228 peripheral blood mononuclear cell (PBMC) specimens, and 179 oropharyngeal (OP) swab specimens obtained from ischemic stroke patients or matched controls. The real-time VD4 assay and one nested PCR each detected C. pneumoniae in a single, but different, PBMC specimen. Eleven of 179 OP specimens (6.1%) showed evidence of the presence of C. pneumoniae in one or more tests. The real-time VD4 assay detected the most positive results of the five assays. We believe that this real-time PCR assay offers advantages over nested PCR assays and may improve the detection of C. pneumoniae in clinical specimens.
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