ArticleJournal of clinical microbiology2002
Quantification of feline herpesvirus 1 DNA in ocular fluid samples of clinically diseased cats by real-time TaqMan PCR.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 109 citations in OpenAlex.
- Evaluation of a novel immunotherapy for treatment of chronic rhinitis in cats.Journal of feline medicine and surgery · 2006Trial
- Antiviral effect of poly(styrene 4-sulfonate) (PSSNa) on feline calicivirus oral infections in cats-field study.The veterinary quarterly · 2026Article
- Isolation of a Variant of the Felid Herpesvirus 1-Live Attenuated Vaccine Strain F2 from Unvaccinated Cats.Intervirology · 2026Article
- Causes of morbidity and mortality in free-ranging Eurasian lynx (Lynx lynx) in Switzerland, 2000-2022.PloS one · 2026Article
- Human herpesvirus 7 epithelial keratitis with geographic-dendritic ulceration.BMC infectious diseases · 2025Article
- Viral Coinfections Potentially Associated with Feline Chronic Gingivostomatitis in Cats with Feline Infectious Peritonitis.Viruses · 2025Article
- Isolation of the feline herpesvirus-1 modified live vaccine strain F2 from one of four cats with dendritic ulcers.Journal of feline medicine and surgery · 2025Article
- A novel feline herpesvirus vector subunit FCV VP1 and FPV VP2 vaccine protects cats against FHV-1 and FPV challenge and induces serum neutralizing antibody responses against FCV.Frontiers in immunology · 2025Article
- Long-term surveillance of the feline leukemia virus in the endangered Iberian lynx (Lynx pardinus) in Andalusia, Spain (2008-2021).Scientific reports · 2024Article
- Hepadnavirus Infection in a Cat with Chronic Liver Disease: A Multi-Disciplinary Diagnostic Approach.Veterinary sciences · 2023Article
- Feline herpesvirus 1 (FHV-1) enters the cell by receptor-mediated endocytosis.Journal of virology · 2023Article
- An Outbreak of Limping Syndrome Associated with Feline Calicivirus.Animals : an open access journal from MDPI · 2023Article
- Pathogenicity and immunogenicity of gI/gE/TK-gene-deleted Felid herpesvirus 1 variants in cats.Virology journal · 2023Article
- Development of immortalized feline respiratory epithelial cells in an air-liquid-interface culture system for feline herpesvirus-1 study.Virus research · 2023Article
- First Report of Isolation and Molecular Characterization of Felid Herpesvirus-1 from Symptomatic Domestic Cats in Egypt.Veterinary sciences · 2022Article
- Quantitative real-time polymerase chain reaction analysis in herpes simplex virus keratitis with and without epithelial involvement.International ophthalmology · 2021Article
- SARS-CoV-2 Infection and Antibody Response in a Symptomatic Cat from Italy with Intestinal B-Cell Lymphoma.Viruses · 2021Article
- Detection and Genome Sequencing of SARS-CoV-2 in a Domestic Cat with Respiratory Signs in Switzerland.Viruses · 2021Article
- Disease Surveillance during the Reintroduction of the Iberian Lynx (Animals : an open access journal from MDPI · 2021Article
- Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A fluorogenic PCR was established for the quantification of feline herpesvirus 1 (FeHV-1) DNA in ocular fluid samples of clinically diseased cats. The new assay was specific for FeHV-1 and sensitive. The 100% detection rate ranged from 0.6 to 6 50% tissue culture infective doses per sample. When spiked samples with known quantities of virus were used, infectious virus titers and quantification of viral DNA by PCR correlated to each other in a linear fashion (R(2) = 0.9858) over a range of 4 orders of magnitude. Within this range, it was possible to calculate the FeHV-1 DNA content from a given infectious dose, and vice versa. The new diagnostic procedure was applied to ocular fluid samples from cats experimentally infected with FeHV-1 and specific FeHV-1-free cats. A good correlation between virus titer and quantitative PCR was observed, although only early in infection. In a second stage, the titer of infectious virus collapsed, while the PCR signal remained high. A constantly decreasing PCR signal accompanied by negative virus isolation was characteristic for a final stage of the infection. Finally, clinical samples from 20 cats that were suspected to suffer from FeHV-1 infection were analyzed. By comparing virus titers and quantitative PCR signals, it was possible to determine the current stage of the ongoing infection. Based on these findings, comparison of the results of consecutive samples allows the tracking of the course of the infection. Therefore, the new method combines the advantages of the two previously established conventional methods, qualitative PCR and virus isolation and titration.
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