ArticleJournal of clinical microbiology2002
Development and evaluation of a quantitative, touch-down, real-time PCR assay for diagnosing Pneumocystis carinii pneumonia.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers, 2 of them syntheses that pooled it.
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Who cites it
56 citing papers in PubMed, 2 syntheses or guidelines pooled it, 172 citations in OpenAlex.
- Meta-analysis of variable-temperature PCR technique performance for diagnosising Schistosoma japonicum infections in humans in endemic areas.PLoS neglected tropical diseases · 2022Pooled it
- The prevalence of laboratory-confirmed Pneumocystis jirovecii in HIV-infected adults in Africa: A systematic review and meta-analysis.Medical mycology · 2021Pooled it
- Preliminary study of molecular identification of Mycobacterium bovis from cow's milk in Lorestan (Iran).Scientific reports · 2024Article
- The Brief Case: False-negativeJournal of clinical microbiology · 2024Article
- Axenic Long-Term Cultivation ofJournal of fungi (Basel, Switzerland) · 2023Article
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- Clinical Utilization of DiaSorin Molecular Polymerase Chain Reaction inOpen forum infectious diseases · 2022Article
- Short Communication: Prevalence ofVeterinary medicine international · 2022Article
- Is It Possible to DifferentiateJournal of fungi (Basel, Switzerland) · 2021Article
- Semiquantitative Real-Time PCR to Distinguish Pneumocystis Pneumonia from Colonization in a Heterogeneous Population of HIV-Negative Immunocompromised Patients.Microbiology spectrum · 2021Article
- Evaluation of the Amplex eazyplex Loop-Mediated Isothermal Amplification Assay for Rapid Diagnosis of Pneumocystis jirovecii Pneumonia.Journal of clinical microbiology · 2020Article
- Is real time PCR preferable to the direct immunofluorescence in the diagnosis of Pneumocystis jirovecii pneumonia in HIV-infected patients?BMC research notes · 2020Article
- Eliminating primer dimers and improving SNP detection using self-avoiding molecular recognition systems.Biology methods & protocols · 2020Article
- Comparative Analysis of the Wako β-Glucan Test and the Fungitell Assay for Diagnosis of Candidemia and Pneumocystis jirovecii Pneumonia.Journal of clinical microbiology · 2018Article
- Inter-Specimen Imbalance of Mitochondrial Gene Copy Numbers Predicts Clustering ofJournal of fungi (Basel, Switzerland) · 2018Article
- Inability to Culture Pneumocystis jirovecii.mBio · 2018Article
- Comparison of three cost effective staining methods for detection ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2017Article
- Comparison of quantitative real-time PCR and direct immunofluorescence for the detection of Pneumocystis jirovecii.PloS one · 2017Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
A rapid (time to completion, <4 h, including DNA extraction) and quantitative touch-down (QTD) real-time diagnostic Pneumocystis carinii PCR assay with an associated internal control was developed, using fluorescence resonance energy transfer (FRET) probes for detection. The touch-down procedure significantly increased the sensitivity of the assay compared to a non-touch-down procedure. Tenfold serial dilutions of a cloned target were used as standards for quantification. P. carinii DNA has been detected in respiratory specimens from patients with P. carinii pneumonia (PCP) and from patients without clinical evidence of PCP. The latter probably represents colonization or subclinical infection. It is logical to hypothesize that quantification might prove helpful in distinguishing between infected and colonized patients: the latter group would have lower copy numbers than PCP patients. A blinded retrospective study of 98 respiratory samples (49 lower respiratory tract specimens and 49 oral washes), from 51 patients with 24 episodes of PCP and 34 episodes of other respiratory disease, was conducted. PCR-positive samples from colonized patients contained a lower concentration of P. carinii DNA than samples from PCP patients: lower respiratory tract samples from PCP and non-PCP patients contained a median of 938 (range, 2.4 to 1,040,000) and 2.6 (range, 0.3 to 248) (P < 0.0004) copies per tube, respectively. Oral washes from PCP and non-PCP patients contained a median of 49 (range, 2.1 to 2,595) and 6.5 (range, 2.2 to 10) (P < 0.03) copies per tube, respectively. These data suggest that this QTD PCR assay can be used to determine if P. carinii is present in respiratory samples and to distinguish between colonization and infection.
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