ArticleJournal of clinical microbiology2002
Identification of Mycobacterium spp. by using a commercial 16S ribosomal DNA sequencing kit and additional sequencing libraries.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- Exploring the bioactive landscape: peptides and non-peptides from the human microbiota.NPJ biofilms and microbiomes · 2025Review
- Diagnosis of nontuberculous mycobacterial infections using genomics and artificial intelligence-machine learning approaches: scope, progress and challenges.Frontiers in microbiology · 2025Review
- Molecular identification of nontuberculous mycobacteria using the rpoB, argH and cya genes analysis.AMB Express · 2022Article
- Parapharyngeal tuberculoid mass: A rare complication of Bacillus Calmette-Guérin therapy for urothelial carcinoma in situ.Clinical case reports · 2021Article
- Application of serial tests for Mycobacterium tuberculosis detection to active lung tuberculosis cases in Indonesia.BMC research notes · 2019Article
- Rapid Identification of Clinically RelevantJournal of clinical microbiology · 2019Article
- Article
- Identification of nontuberculous mycobacteria using multilocous sequence analysis of 16S rRNA, hsp65, and rpoB.Journal of clinical laboratory analysis · 2018Article
- [Visualized detection for mycobacterium tuberculosis using loop-mediated isothermal amplification assay].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2017Article
- [Identification ofZhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2016Article
- Molecular Mycobacteriology and Expansion in Disease Diagnosis.Indian journal of clinical biochemistry : IJCB · 2016Review
- Extensively drug-resistant tuberculosis in a young child after travel to India.The Lancet. Infectious diseases · 2015Review
- Absence of a functional erm gene in isolates of Mycobacterium immunogenum and the Mycobacterium mucogenicum group, based on in vitro clarithromycin susceptibility.Journal of clinical microbiology · 2015Article
- Advantages of using matrix-assisted laser desorption ionization-time of flight mass spectrometry as a rapid diagnostic tool for identification of yeasts and mycobacteria in the clinical microbiological laboratory.Journal of clinical microbiology · 2013Article
- Bacterial classification of fish-pathogenic Mycobacterium species by multigene phylogenetic analyses and MALDI Biotyper identification system.Marine biotechnology (New York, N.Y.) · 2013Article
- Evaluation of hsp65 nested PCR-restriction analysis (PRA) for diagnosing tuberculosis in a high burden country.BioMed research international · 2013Article
- Increased incidence of cutaneous nontuberculous mycobacterial infection, 1980 to 2009: a population-based study.Mayo Clinic proceedings · 2013Article
- Rapid and accurate identification of Mycobacterium tuberculosis complex and common non-tuberculous mycobacteria by multiplex real-time PCR targeting different housekeeping genes.Current microbiology · 2012Article
- Tuberculosis diagnostics: Challenges and opportunities.Lung India : official organ of Indian Chest Society · 2012Article
- Biochip system for rapid and accurate identification of mycobacterial species from isolates and sputum.Journal of clinical microbiology · 2010Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Current methods for identification of Mycobacterium spp. rely upon time-consuming phenotypic tests, mycolic acid analysis, and narrow-spectrum nucleic acid probes. Newer approaches include PCR and sequencing technologies. We evaluated the MicroSeq 500 16S ribosomal DNA (rDNA) bacterial sequencing kit (Applied Biosystems, Foster City, Calif.) for its ability to identify Mycobacterium isolates. The kit is based on PCR and sequencing of the first 500 bp of the bacterial rRNA gene. One hundred nineteen mycobacterial isolates (94 clinical isolates and 25 reference strains) were identified using traditional phenotypic methods and the MicroSeq system in conjunction with separate databases. The sequencing system gave 87% (104 of 119) concordant results when compared with traditional phenotypic methods. An independent laboratory using a separate database analyzed the sequences of the 15 discordant samples and confirmed the results. The use of 16S rDNA sequencing technology for identification of Mycobacterium spp. provides more rapid and more accurate characterization than do phenotypic methods. The MicroSeq 500 system simplifies the sequencing process but, in its present form, requires use of additional databases such as the Ribosomal Differentiation of Medical Microorganisms (RIDOM) to precisely identify subtypes of type strains and species not currently in the MicroSeq library.
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