ArticleThe EMBO journal2002
Contrasting requirements for ubiquitylation during Fc receptor-mediated endocytosis and phagocytosis.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 92 citations in OpenAlex.
- Monitoring Phosphoinositide Fluxes and Effectors During Leukocyte Chemotaxis and Phagocytosis.Frontiers in cell and developmental biology · 2021Review
- The Human FcγRII (CD32) Family of Leukocyte FcR in Health and Disease.Frontiers in immunology · 2019Review
- Multiple Variables at the Leukocyte Cell Surface Impact Fc γ Receptor-Dependent Mechanisms.Frontiers in immunology · 2019Review
- Involvement of the p62/NRF2 signal transduction pathway on erythrophagocytosis.Scientific reports · 2017Article
- Antibody-Mediated Internalization of Infectious HIV-1 Virions Differs among Antibody Isotypes and Subclasses.PLoS pathogens · 2016Article
- Proteasome Inhibition Suppresses Dengue Virus Egress in Antibody Dependent Infection.PLoS neglected tropical diseases · 2015Article
- Actin cytoskeleton reorganization by Syk regulates Fcγ receptor responsiveness by increasing its lateral mobility and clustering.Developmental cell · 2014Article
- Regulation of fc receptor endocytic trafficking by ubiquitination.Frontiers in immunology · 2014Review
- FcγRIIa requires lipid rafts, but not co-localization into rafts, for effector function.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2013Article
- E3 ubiquitin ligase NKLAM is a macrophage phagosome protein and plays a role in bacterial killing.Cellular immunology · 2012Article
- IgG keeps virulent Salmonella from evading dendritic cell uptake.Immunology · 2012Article
- Porcine sialoadhesin (CD169/Siglec-1) is an endocytic receptor that allows targeted delivery of toxins and antigens to macrophages.PloS one · 2011Article
- Internalization of IgG-coated targets results in activation and secretion of soluble CD40 ligand and RANTES by human platelets.Clinical and vaccine immunology : CVI · 2011Article
- Regulation of human neutrophil Fcγ receptor IIa by C5a receptor promotes inflammatory arthritis in mice.Arthritis and rheumatism · 2011Article
- Article
- Divergent intracellular sorting of Fc{gamma}RIIA and Fc{gamma}RIIB2.The Journal of biological chemistry · 2010Article
- Analysis of FcgammaRIIA cytoplasmic tail requirements in signaling for serotonin secretion: evidence for an ITAM-dependent, PI3K-dependent pathway.Scandinavian journal of immunology · 2010Article
- Differential requirement of lipid rafts for FcγRIIA mediated effector activities.Cellular immunology · 2010Article
- CD36 and TLR interactions in inflammation and phagocytosis: implications for malaria.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Francisella tularensis induces ubiquitin-dependent major histocompatibility complex class II degradation in activated macrophages.Infection and immunity · 2009Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fc receptors on leukocytes mediate internalization of antibody-containing complexes. Soluble immune complexes are taken up by endocytosis, while large antibody-opsonized particles are internalized by phagocytosis. We investigated the role of ubiquitylation in internalization of the human FcgammaRIIA receptor by endocytosis and phagocytosis. A fusion of FcgammaRIIA to green fluorescent protein (GFP) was expressed in ts20 cells, which bear a temperature-sensitive mutation in the E1 ubiquitin-activating enzyme. Uptake of soluble IgG complexes mediated by FcgammaRIIA-GFP was blocked by incubation at the restrictive temperature, indicating that endocytosis requires ubiquitylation. In contrast, phagocytosis and phagosomal maturation were largely unaffected when ubiquitylation was impaired. FcgammaRIIA-GFP was ubiquitylated in response to receptor cross-linking. Elimination of the lysine residues present in the cytoplasmic domain of FcgammaRIIA impaired endocytosis, but not phagocytosis. The proteasomal inhibitor clasto-lactacystin beta-lactone strongly inhibited endocytosis, but did not affect phagocytosis. These studies demonstrate a role for ubiquitylation in the endocytosis of immune receptors, and reveal fundamental differences in the mechanisms underlying internalization of a single receptor depending on the size or multiplicity of the ligand complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.