ArticleApplied and environmental microbiology2002
Transcriptional and proteomic analysis of a ferric uptake regulator (fur) mutant of Shewanella oneidensis: possible involvement of fur in energy metabolism, transcriptional regulation, and oxidative stress.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed, 172 citations in OpenAlex.
- Effects of RyhB on Fur regulation of cytochromeApplied and environmental microbiology · 2026Article
- Article
- Extracellular catalysis of environmental substrates by Shewanella oneidensis MR-1 occurs via active sites on the C-terminal domains of MtrC.Protein science : a publication of the Protein Society · 2025Article
- Robust measurement of microbial reduction of graphene oxide nanoparticles using image analysis.Applied and environmental microbiology · 2025Article
- Biological and Chemical Processes of Nitrate Reduction and Ferrous Oxidation Mediated byMicroorganisms · 2024Article
- Structural Perspectives on Metal Dependent Roles of Ferric Uptake Regulator (Fur).Biomolecules · 2024Review
- Compensatory evolution of chromosomes and plasmids counteracts the plasmid fitness cost.Ecology and evolution · 2024Article
- The Fur-like regulatory protein MAP3773c modulates key metabolic pathways in Mycobacterium avium subsp. paratuberculosis under in-vitro iron starvation.Scientific reports · 2024Article
- The Mutation of the DNA-Binding Domain of Fur Protein Enhances the Pathogenicity ofMicroorganisms · 2023Article
- Biochemical characterization of ferric uptake regulator (Fur) from Aliivibrio salmonicida. Mapping the DNA sequence specificity through binding studies and structural modelling.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2020Article
- Free Rather Than Total Iron Content Is Critically Linked to the Fur Physiology inFrontiers in microbiology · 2020Article
- A Novel Small RNA on theFrontiers in microbiology · 2020Article
- Periodic and coordinated gene expression between a diazotroph and its diatom host.The ISME journal · 2019Article
- Dissociation between Iron and Heme Biosyntheses Is Largely Accountable for Respiration Defects ofApplied and environmental microbiology · 2018Article
- MgtE Homolog FicI Acts as a Secondary Ferrous Iron Importer in Shewanella oneidensis Strain MR-1.Applied and environmental microbiology · 2018Article
- The Small RNA RyhB Is a Regulator of Cytochrome Expression inFrontiers in microbiology · 2018Article
- Emerging patterns of plasmid-host coevolution that stabilize antibiotic resistance.Scientific reports · 2017Article
- The Outer Membrane Protein OmpW EnhancedFrontiers in microbiology · 2017Article
- Secreted Citrate Serves as Iron Carrier for the Marine PathogenFrontiers in cellular and infection microbiology · 2017Article
- Redox Linked Flavin Sites in Extracellular Decaheme Proteins Involved in Microbe-Mineral Electron Transfer.Scientific reports · 2015Article
18 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The iron-directed, coordinate regulation of genes depends on the fur (ferric uptake regulator) gene product, which acts as an iron-responsive, transcriptional repressor protein. To investigate the biological function of a fur homolog in the dissimilatory metal-reducing bacterium Shewanella oneidensis MR-1, a fur knockout strain (FUR1) was generated by suicide plasmid integration into this gene and characterized using phenotype assays, DNA microarrays containing 691 arrayed genes, and two-dimensional polyacrylamide gel electrophoresis. Physiological studies indicated that FUR1 was similar to the wild-type strain when they were compared for anaerobic growth and reduction of various electron acceptors. Transcription profiling, however, revealed that genes with predicted functions in electron transport, energy metabolism, transcriptional regulation, and oxidative stress protection were either repressed (ccoNQ, etrA, cytochrome b and c maturation-encoding genes, qor, yiaY, sodB, rpoH, phoB, and chvI) or induced (yggW, pdhC, prpC, aceE, fdhD, and ppc) in the fur mutant. Disruption of fur also resulted in derepression of genes (hxuC, alcC, fhuA, hemR, irgA, and ompW) putatively involved in iron uptake. This agreed with the finding that the fur mutant produced threefold-higher levels of siderophore than the wild-type strain under conditions of sufficient iron. Analysis of a subset of the FUR1 proteome (i.e., primarily soluble cytoplasmic and periplasmic proteins) indicated that 11 major protein species reproducibly showed significant (P < 0.05) differences in abundance relative to the wild type. Protein identification using mass spectrometry indicated that the expression of two of these proteins (SodB and AlcC) correlated with the microarray data. These results suggest a possible regulatory role of S. oneidensis MR-1 Fur in energy metabolism that extends the traditional model of Fur as a negative regulator of iron acquisition systems.
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