ArticleApplied and environmental microbiology2002
Highly different levels of natural transformation are associated with genomic subgroups within a local population of Pseudomonas stutzeri from soil.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 84 citations in OpenAlex.
- Natural competence in the bacterial pathogen Xylella fastidiosa varies across genotypes and is associated with adhesins.PLoS pathogens · 2025Article
- Impact of Natural Transformation on the Acquisition of Novel Genes in Bacteria.Molecular biology and evolution · 2025Article
- Intragenomic conflicts with plasmids and chromosomal mobile genetic elements drive the evolution of natural transformation within species.PLoS biology · 2024Article
- Mechanism of antibacterial resistance, strategies and next-generation antimicrobials to contain antimicrobial resistance: a review.Frontiers in pharmacology · 2024Review
- Experimental evolution of the megaplasmid pMPPla107 inPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
- Natural Transformation as a Mechanism of Horizontal Gene Transfer inPathogens (Basel, Switzerland) · 2021Article
- Genomic Background Governs Opposing Responses to Nalidixic Acid upon Megaplasmid Acquisition inmSphere · 2021Article
- Comparative Genomics ofFrontiers in microbiology · 2021Article
- Complete genome sequencing and comparative genome characterization of the lignocellulosic biomass degrading bacteriumBiotechnology reports (Amsterdam, Netherlands) · 2020Article
- Non-antibiotic pharmaceuticals enhance the transmission of exogenous antibiotic resistance genes through bacterial transformation.The ISME journal · 2020Article
- Diverse conjugative elements silence natural transformation inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Isolation of nitrate-reducing bacteria from an offshore reservoir and the associated biosurfactant production.RSC advances · 2018Article
- Dynamics of a microbial community during an effective boost MEOR trial using high-throughput sequencing.RSC advances · 2018Article
- Addiction of Hypertransformable Pneumococcal Isolates to Natural Transformation for In Vivo Fitness and Virulence.Infection and immunity · 2016Article
- Antimicrobial resistance of Pseudomonas spp. isolated from wastewater and wastewater-impacted marine coastal zone.Environmental science and pollution research international · 2015Article
- Genomovar assignment of Pseudomonas stutzeri populations inhabiting produced oil reservoirs.MicrobiologyOpen · 2014Article
- Complete Genome Sequence of the Highly Transformable Pseudomonas stutzeri Strain 28a24.Genome announcements · 2014Article
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- Significant variation in transformation frequency in Streptococcus pneumoniae.The ISME journal · 2013Article
- Genome sequence of Pseudomonas stutzeri strain JM300 (DSM 10701), a soil isolate and model organism for natural transformation.Journal of bacteriology · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A highly sensitive and specific PCR-based method of monitoring 16S rRNA genes of Pseudomonas stutzeri was developed for searching P. stutzeri DNA in environmental samples. This monitoring was combined with a reliable and sensitive method for isolating P. stutzeri colony formers from soil and sediment, depending on their utilization of ethylene glycol, starch, and maltose. With these techniques, P. stutzeri populations (n = 2 to 170) were obtained from five of six sites giving positive PCR signals (including three marine sediment and two soil samples). The phylogenetic positions of isolates from the five sites, based on their 16S ribosomal DNA sequences, indicated that the environmental isolates were affiliated with different genomovars of P. stutzeri. Using the broad-host-range plasmid pNS1 with kanamycin and gentamicin resistance determinants as the transforming DNA, naturally transformable strains were identified among the isolates from all sites. For one population from soil, the genetic relationship of the 120 members was determined by randomly amplified polymorphic DNA-PCR with three PCR primers. Among the population members which are taxonomically closely related as determined by 16S sequence comparisons of group representatives, a rather high genetic diversity and a characteristic clustering into subgroups were found. Remarkably, within the population, nontransformability and different levels of transformability (a frequency between about 10(-9) and 10(-4) per cell) were often associated with distinct genetic subgroups. It is concluded that transformability is widespread among environmental P. stutzeri strains and that its specific level is a heritable trait that may vary strongly within a local population.
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