ArticleApplied and environmental microbiology2002
Calnexin overexpression increases manganese peroxidase production in Aspergillus niger.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 78 citations in OpenAlex.
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- Exploration of the Strategy for Improving the Expression of Heterologous Sweet Protein Monellin inJournal of fungi (Basel, Switzerland) · 2023Article
- Focus and Insights into the Synthetic Biology-Mediated Chassis of Economically Important Fungi for the Production of High-Value Metabolites.Microorganisms · 2023Review
- Unlocking the magic in mycelium: Using synthetic biology to optimize filamentous fungi for biomanufacturing and sustainability.Materials today. Bio · 2023Article
- Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion.Frontiers in bioengineering and biotechnology · 2020Review
- Degradation of Four Major Mycotoxins by Eight Manganese Peroxidases in Presence of a Dicarboxylic Acid.Toxins · 2019Article
- Protein profile in Aspergillus nidulans recombinant strains overproducing heterologous enzymes.Microbial biotechnology · 2018Article
- Ultrastructural features of the early secretory pathway in Trichoderma reesei.Current genetics · 2016Article
- Like iron in the blood of the people: the requirement for heme trafficking in iron metabolism.Frontiers in pharmacology · 2014Review
- Coal Depolymerising Activity and Haloperoxidase Activity of Mn Peroxidase from Fomes durissimus MTCC-1173.Bioinorganic chemistry and applications · 2011Article
- Microbial degradation of lignin: how a bulky recalcitrant polymer is efficiently recycled in nature and how we can take advantage of this.Microbial biotechnology · 2009Review
- Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview.Microbial cell factories · 2008Article
- Engineering eukaryotic protein factories.Biotechnology letters · 2008Review
- Spatial differentiation in the vegetative mycelium of Aspergillus niger.Eukaryotic cell · 2007Article
- Increase of calnexin gene dosage boosts the secretion of heterologous proteins by Hansenula polymorpha.FEMS yeast research · 2007Article
- UPR-independent dithiothreitol stress-induced genes in Aspergillus niger.Molecular genetics and genomics : MGG · 2005Article
- Modulation of Aspergillus awamori thaumatin secretion by modification of bipA gene expression.Applied and environmental microbiology · 2004Article
- Cell viability and secretion of active proteins in Schizosaccharomyces pombe do not require the chaperone function of calnexin.The Biochemical journal · 2004Article
- Improvement of foreign-protein production in Aspergillus niger var. awamori by constitutive induction of the unfolded-protein response.Applied and environmental microbiology · 2003Article
- Homologous expression of Phanerochaete chrysosporium manganese peroxidase, using bialaphos resistance as a dominant selectable marker.Current genetics · 2003Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heme-containing peroxidases from white rot basidiomycetes, in contrast to most proteins of fungal origin, are poorly produced in industrial filamentous fungal strains. Factors limiting peroxidase production are believed to operate at the posttranslational level. In particular, insufficient availability of the prosthetic group which is required for peroxidase biosynthesis has been proposed to be an important bottleneck. In this work, we analyzed the role of two components of the secretion pathway, the chaperones calnexin and binding protein (BiP), in the production of a fungal peroxidase. Expression of the Phanerochaete chrysosporium manganese peroxidase (MnP) in Aspergillus niger resulted in an increase in the expression level of the clxA and bipA genes. In a heme-supplemented medium, where MnP was shown to be overproduced to higher levels, induction of clxA and bipA was also higher. Overexpression of these two chaperones in an MnP-producing strain was analyzed for its effect on MnP production. Whereas bipA overexpression seriously reduced MnP production, overexpression of calnexin resulted in a four- to fivefold increase in the extracellular MnP levels. However, when additional heme was provided in the culture medium, calnexin overexpression had no synergistic effect on MnP production. The possible function of these two chaperones in MnP maturation and production is discussed.
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