ArticleApplied and environmental microbiology2002
Characterization and application of xylene monooxygenase for multistep biocatalysis.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 107 citations in OpenAlex.
- Evolution of Methods for the Oxidation of Primary Alcohols to Carboxylic Acids: From Metal Oxides to Biocatalysis.JACS Au · 2026Review
- Comparative Genomic Analysis and BTEX Degradation Pathways of a ThermotolerantJournal of microbiology and biotechnology · 2023Article
- Biodegradation of p-xylene-a comparison of three psychrophilic Pseudomonas strains through the lens of gene expression.Environmental science and pollution research international · 2022Article
- Subcellular Architecture of themBio · 2021Article
- BTEX biodegradation by Bacillus amyloliquefaciens subsp. plantarum W1 and its proposed BTEX biodegradation pathways.Scientific reports · 2020Article
- Selective oxidation of aliphatic C-H bonds in alkylphenols by a chemomimetic biocatalytic system.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Applied catastrophic phase inversion: a continuous non-centrifugal phase separation step in biphasic whole-cell biocatalysis.Journal of industrial microbiology & biotechnology · 2016Article
- Making variability less variable: matching expression system and host for oxygenase-based biotransformations.Journal of industrial microbiology & biotechnology · 2015Article
- Bioconversion of α-pinene by a novel cold-adapted fungus Chrysosporium pannorum.Journal of industrial microbiology & biotechnology · 2015Article
- On the (Un)greenness of Biocatalysis: Some Challenging Figures and Some Promising Options.Frontiers in microbiology · 2015Review
- Engineering of Pseudomonas taiwanensis VLB120 for constitutive solvent tolerance and increased specific styrene epoxidation activity.Applied and environmental microbiology · 2014Article
- Integrated organic-aqueous biocatalysis and product recovery for quinaldine hydroxylation catalyzed by living recombinant Pseudomonas putida.Journal of industrial microbiology & biotechnology · 2012Article
- Response of Pseudomonas putida KT2440 to increased NADH and ATP demand.Applied and environmental microbiology · 2011Article
- Cell physiology rather than enzyme kinetics can determine the efficiency of cytochrome P450-catalyzed C-H-oxyfunctionalization.Journal of industrial microbiology & biotechnology · 2011Article
- Regioselective aromatic hydroxylation of quinaldine by water using quinaldine 4-oxidase in recombinant Pseudomonas putida.Journal of industrial microbiology & biotechnology · 2011Article
- Pyruvate kinase-deficient Escherichia coli exhibits increased plasmid copy number and cyclic AMP levels.Journal of bacteriology · 2009Article
- NADH availability limits asymmetric biocatalytic epoxidation in a growing recombinant Escherichia coli strain.Applied and environmental microbiology · 2008Article
- Protein engineering of toluene monooxygenases for synthesis of chiral sulfoxides.Applied and environmental microbiology · 2008Article
- Suitability of recombinant Escherichia coli and Pseudomonas putida strains for selective biotransformation of m-nitrotoluene by xylene monooxygenase.Applied and environmental microbiology · 2005Article
- Toluene 3-monooxygenase of Ralstonia pickettii PKO1 is a para-hydroxylating enzyme.Journal of bacteriology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Xylene monooxygenase of Pseudomonas putida mt-2 catalyzes multistep oxidations of one methyl group of toluene and xylenes. Recombinant Escherichia coli expressing the monooxygenase genes xylM and xylA catalyzes the oxygenation of toluene, pseudocumene, the corresponding alcohols, and the corresponding aldehydes, all by a monooxygenation type of reaction (B. Bühler, A. Schmid, B. Hauer, and B. Witholt, J. Biol. Chem. 275:10085-10092, 2000). Using E. coli expressing xylMA, we investigated the kinetics of this one-enzyme three-step biotransformation. We found that unoxidized substrates like toluene and pseudocumene inhibit the second and third oxygenation steps and that the corresponding alcohols inhibit the third oxygenation step. These inhibitions might promote the energetically more favorable alcohol and aldehyde dehydrogenations in the wild type. Growth of E. coli was strongly affected by low concentrations of pseudocumene and its products. Toxicity and solubility problems were overcome by the use of a two-liquid-phase system with bis(2-ethylhexyl)phthalate as the carrier solvent, allowing high overall substrate and product concentrations. In a fed-batch-based two-liquid-phase process with pseudocumene as the substrate, we observed the consecutive accumulation of aldehyde, acid, and alcohol. Our results indicate that, depending on the reaction conditions, product formation could be directed to one specific product.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.