ArticleWorld journal of gastroenterology2001
Effects of hypoxia, hyperoxia on the regulation of expression and activity of matrix metalloproteinase-2 in hepatic stellate cells.
Article in World journal of gastroenterology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Effects of heparin on liver fibrosis in patients with chronic hepatitis B.World journal of gastroenterology · 2003Trial
- RNA sequencing reveals widespread transcriptome changes in a renal carcinoma cell line.Oncotarget · 2018Article
- Changes in the expression of Col IV, gelatinase and TIMP-1 in oral leukoplakia.International journal of clinical and experimental pathology · 2017Article
- In vivo imaging of membrane type-1 matrix metalloproteinase with a novel activatable near-infrared fluorescence probe.Cancer science · 2014Article
- Hyperoxia accelerates progression of hepatic fibrosis by up-regulation of transforming growth factor-β expression.World journal of gastroenterology · 2014Article
- Reactive oxygen species released from hypoxic hepatocytes regulates MMP-2 expression in hepatic stellate cells.International journal of molecular sciences · 2011Article
- Oxygen tension modulates neurite outgrowth in PC12 cells through a mechanism involving HIF and VEGF.Journal of molecular neuroscience : MN · 2010Article
- Role of matrix metalloprotease-9 in hyperoxic injury in developing lung.American journal of physiology. Lung cellular and molecular physiology · 2008Article
- Graded hypoxia modulates the invasive potential of HT1080 fibrosarcoma and MDA MB231 carcinoma cells.Clinical & experimental metastasis · 2008Article
- Matrix metalloproteinases 2 and 9 in oral squamous cell carcinomas: manifestation and localization of their activity.Journal of cancer research and clinical oncology · 2005Article
- Antiproliferative and proapoptotic effects of somatostatin on activated hepatic stellate cells.World journal of gastroenterology · 2004Article
- Increased expression of MMP-2 and MMP-9 in esophageal squamous cell carcinoma.Journal of cancer research and clinical oncology · 2004Article
- Inhibitory effects of docetaxel on expression of VEGF, bFGF and MMPs of LS174T cell.World journal of gastroenterology · 2003Article
- Effects of tetrandrine on calcium and potassium currents in isolated rat hepatocytes.World journal of gastroenterology · 2003Article
- Estrogen reduces CCL4- induced liver fibrosis in rats.World journal of gastroenterology · 2002Article
- Protective effect of estradiol on hepatocytic oxidative damage.World journal of gastroenterology · 2002Article
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo study the effects of hypoxia, hyperoxia on the regulation of expression and activity of matrix metalloproteinase-2 (MMP-2) in hepatic stellate cells (HSC).
methodsThe expressions of MMP-2, tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) and membrane type matrix metalloproteinase-1 (MT1-MMP) in cultured rat HSC were detected by immunocytochemistry (ICC) and in situ hybridization (ISH). The contents of MMP-2 and TIMP-2 in culture supernatant were detected with ELISA and the activity of MMP-2 in supernatant was revealed by zymography.
resultsIn the situation of hypoxia for 12h, the expression of MMP-2 protein was enhanced (hypoxia group positive indexes: 5.7 +/- 2.0, n=10; control: 3.2 +/- 1.0, n = 7; P<0.05), while TIMP-2 protein was decreased in HSC (hypoxia group positive indexes: 2.5 +/- 0.7, n = 10; control: 3.6 +/- 1.0, n = 7; P < 0.05), and the activity (total A) of MMP-2 in supernatant declined obviously (hypoxia group: 7.334 +/- 1.922, n = 9; control: 17.277 +/- 7.424, n = 11; P < 0.01). Compared the varied duration of hypoxia, the changes of expressions including mRNA and protein level as well as activity of MMP-2 were most notable in 6h group. The highest value(A(hypoxia)-A(control)) of the protein and the most intense signal of mRNA were in the period of hypoxia for 6h, along with the lowest activity of MMP-2. In the situation of hyperoxia for 12h, the contents (A(450)) of MMP-2 and TIMP-2 in supernatant were both higher than those in the control, especially the TIMP-2 (hyperoxia group: 0.0499 +/- 0.0144, n = 16; control: 0.0219 +/- 0.0098, n = 14; P < 0.01), and so was the activity of MMP-2 (hyperoxia group: 5.252 +/- 0.771, n = 14; control: 4.304 +/- 1.083, n = 12; P < 0.05), and the expression of MT1-MMP was increased.
conclusionHSC is sensitive to the oxygen, hypoxia enhances the expression of MMP-2 and the effect is more marked at the early stage; hyperoxia mainly raises the activity of MMP-2.
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