Evidence map›Paper›PMID 11819822›Full record

ArticleWorld journal of gastroenterology2001

Extraction and purification of TGFbeta and its effect on the induction of apoptosis of hepatocytes.

X H Si, L J Yang

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in World journal of gastroenterology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.2field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 21 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

X H SiResearch Institute of Stomatology, the Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China.
L J Yang
Shanghai Ninth People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimTo extract and purify the transforming growth factor beta (TGF beta), and to demonstrate its biological activity in vivo and induction of apoptosis of hepatocytes in vitro.

methodsTGF beta was isolated from fresh bovine platelets by acid/ethanol extraction method and purified with ion exchange and gel chromatography. The extracted TGF beta was injected subcutaneously to mice, and its biological activity in vitro was observed 72 hrs post-injection by HE staining. The morphological changes were observed by HE staining and the occurrence of apoptosis was detected by TUNEL method after the human normal hepatic cell line QZG was treated with 8 microg.(-1)TGFbeta for 12 hrs in vitro.

resultsThe molecular mass 25 ku TGFbeta protein was successfully extracted. It was able to induce localized granulation tissue formation in vitro. TGF beta-treated hepatocytes showed obvious apoptotic morphological changes, including the pyknosis and dense-stained nuclei and cytoplasm, the fragmentary, annular or crescent nuclei, and the "bubbling" cytoplasm. Moreover, its apoptotic rate was significantly higher than that of the control group (P<0.05).

conclusionBiological active TGF beta protein is extracted and purified successfully from bovine platelets, and it is able to induce the apoptosis of hepatocytes.

Indexed as

AnimalsApoptosisBlood PlateletsCattleCell LineHepatocytesHumansInjections, SubcutaneousMaleMiceMice, Inbred BALB CSignal TransductionTransforming Growth Factor betaTransforming Growth Factor beta

Identifiers

PMID11819822
PMCPMC4688666
OpenAlexW864620230

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.