ArticleWorld journal of gastroenterology2001
Expression of vascular endothelial growth factor and its role in oncogenesis of human gastric carcinoma.
Article in World journal of gastroenterology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 47 citations in OpenAlex.
- Calreticulin regulates vascular endothelial growth factor-A mRNA stability in gastric cancer cells.PloS one · 2019Article
- The VEGF -634G>C promoter polymorphism is associated with risk of gastric cancer.BMC gastroenterology · 2009Article
- Polymorphisms of TGFB1 and VEGF genes and survival of patients with gastric cancer.Journal of experimental & clinical cancer research : CR · 2009Article
- Histopathological predictor for regional lymph node metastasis in gastric cancer.Virchows Archiv : an international journal of pathology · 2009Article
- Significance of vascular endothelial growth factor expression and its correlation with inducible nitric oxide synthase in gastric cancer.World journal of gastroenterology · 2004Article
- Construction of prokaryotic expression system of 2 148-bp fragment from cagA gene and detection of cagA gene, CagA protein in Helicobacter pylori isolates and its antibody in sera of patients.World journal of gastroenterology · 2004Article
- Effects of vitamin E succinate on the expression of Fas and PCNA proteins in human gastric carcinoma cells and its clinical significance.World journal of gastroenterology · 2004Article
- Construction of a prokaryotic expression system of vacA gene and detection of vacA gene, VacA protein in Helicobacter pylori isolates and ant-VacA antibody in patients' sera.World journal of gastroenterology · 2004Article
- Construction of prokaryotic expression system of ureB gene from a clinical Helicobacter pylori strain and identification of the recombinant protein immunity.World journal of gastroenterology · 2004Article
- Establishment of Helicobacter pylori infection model in Mongolian gerbils.World journal of gastroenterology · 2004Article
- Construction and analysis of SSH cDNA library of human vascular endothelial cells related to gastrocarcinoma.World journal of gastroenterology · 2003Article
- Construction of expression systems for flaA and flaB genes of Helicobacter pylori and determination of immunoreactivity and antigenicity of recombinant proteins.World journal of gastroenterology · 2003Article
- Expression of MTLC gene in gastric carcinoma.World journal of gastroenterology · 2003Article
- Inhibitory effects of docetaxel on expression of VEGF, bFGF and MMPs of LS174T cell.World journal of gastroenterology · 2003Article
- Effects of carbon dioxide and nitrogen on adhesive growth and expressions of E-cadherin and VEGF of human colon cancer cell CCL-228.World journal of gastroenterology · 2003Article
- Association of VCAM-1 overexpression with oncogenesis, tumor angiogenesis and metastasis of gastric carcinoma.World journal of gastroenterology · 2003Article
- Prognostic significance of expression of cyclooxygenase-2 and vascular endothelial growth factor in human gastric carcinoma.World journal of gastroenterology · 2003Article
- Expression of NGF family and their receptors in gastric carcinoma: a cDNA microarray study.World journal of gastroenterology · 2003Article
- cDNA suppression subtraction library for screening down-regulated genes in gastric carcinoma.World journal of gastroenterology · 2003Article
- Construction of hpaA gene from a clinical isolate of Helicobacter pylori and identification of fusion protein.World journal of gastroenterology · 2003Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo establish the role of vascular endothelial growth factor (VEGF) in the oncogenesis of human gastric carcinoma more directly.
methodsThe expression of VEGF and its receptor kinase-domain insert containing receptor (KDR) in human gastric cancer tissue were observed by immunohistochemical staining. VEGF levels were manipulated in human gastric cancer cell using eukaryotic expression constructs designed to express the complete VEGF(165) complimentary DNA in either the sense or antisense orientation. The biological changes of the cells were observed in which VEGF was up-regulated or down-regulated.
resultsVEGF-positive rate was 50%, and VEGF was mainly localized in the cytoplasm and membrane of the tumor cells, while KDR was mainly located in the membrane of vascular endothelial cells in gastric cancer tissues and peri-cancerous tissue. In 2 cases of 50 specimens, the gastric cancer cells expressed KDR, localized in both the cytoplasm and membrane. Introduction of VEGF(165) antisense into human gastric cancer cells (SGC-7901, immunofluorescence intensity, 31.6%)) resulted in a significant reduction in VEGF-specific messenger RNA and total and cell surface VEGF protein (immunofluorescence intensity, 8.9%) (P<0.05). Conversely, stable integration of VEGF(165) in the sense orientation resulted in an increase in cellular and cell surface VEGF (immunofluorescence intensity, 75.4%) (P<0.05). Lowered VEGF levels were associated with a marked decrease in the growth of nude mouse xenografted tumor (at 33 days postimplantation, tumor volume: 345.40 +/- 136.31 mm3)(P<0.05 vs control SGC-7901 group: 1534.40 +/- 362.88 mm3), whereas up-regulation of VEGF resulted in increased xenografted tumor size (at 33 days postimplantation, tumor volume: 2350.50 +/- 637.70 mm3) (P<0.05 vs control SGC-7901 group).
conclusionThis study provides direct evidence that VEGF plays an important role in the oncogenesis of human gastric cancer.
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