ArticleWorld journal of gastroenterology2001
Virulence and potential pathogenicity of coccoid Helicobacter pylori induced by antibiotics.
Article in World journal of gastroenterology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Seven-day quintuple regimen as a rescue therapy for Helicobacter pylori eradication.World journal of gastroenterology · 2015Trial
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- Comparison of three methods for generating the coccoid form of Helicobacter pylori and proteomic analysis.BMC microbiology · 2024Article
- The Puzzle of Coccoid Forms ofAntibiotics (Basel, Switzerland) · 2020Review
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- Morphology ofPrzeglad gastroenterologiczny · 2018Review
- Evaluation of Invasive and Noninvasive Methods for the Diagnosis of Helicobacter Pylori InfectionAsian Pacific journal of cancer prevention : APJCP · 2016Article
- Morphological and Bactericidal Effects of Different Antibiotics on Helicobacter pylori.Jundishapur journal of microbiology · 2014Article
- Assessment of cagE and babA mRNA expression during morphological conversion of Helicobacter pylori from spiral to coccoid.Current microbiology · 2013Article
- The bactericidal activity of carbon monoxide-releasing molecules against Helicobacter pylori.PloS one · 2013Article
- Antibacterial activities of liposomal linolenic acids against antibiotic-resistant Helicobacter pylori.Molecular pharmaceutics · 2012Article
- Successful isolation of Helicobacter pylori after prolonged incubation from a patient with failed eradication therapy.World journal of gastroenterology · 2009Article
- Urease activity and urea gene sequencing of coccoid forms of H. pylori induced by different factors.Current microbiology · 2008Article
- Relationship of Helicobacter pylori to Bcl-2 family expression, DNA content, and pathological characteristics of gastric cancer.International journal of gastrointestinal cancer · 2005Article
- Helicobacter pylori L-form and patients with chronic gastritis.World journal of gastroenterology · 2004Article
- Expression of Helicobacter pylori Hsp60 protein and its immunogenicity.World journal of gastroenterology · 2003Article
- cagA and vacA genotype of Helicobacter pylori associated with gastric diseases in Xi'an area.World journal of gastroenterology · 2003Article
- Recombinant Helicobacter pylori catalase.World journal of gastroenterology · 2003Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo explore the virulence and the potential pathogenicity of coccoid Helicobacter pylori (H. pylori) transformed from spiral form by exposure to antibiotic.
methodsThree strains of H. pylori, isolated from gastric biopsy specimens of confirmed peptic ulcer, were converted from spiral into coccoid from by exposure to metronidazole. Both spiral and coccoid form of H. pylori were tested for the urease activity, the adherence to Hep-2 cells and the vacuolating cytotoxicity to Hela cells, and the differences of the protein were analysed by SDS-PAGE and Western blot. The mutation of the genes including ureA, ureB,hpaA, vacA and cagA, related with virulence, was detected by means of PCR and PCR-SSCP.
resultsIn the coccoid H. pylori,the urease activity, the adherence to Hep-2 cells and the vacuolating cytotoxicity to Hela cells all decreased. In strain F44, the rate and index of adherence reduced from 70.0% +/- 5.3% to 33% +/- 5.1% and from 2.6 +/- 0.4 to 0.96 +/- 0.3 (P < 0.01), respectively. The invasion of coccoid H. pylori into Hep-2 cell could be seen under electronmicroscope. SDS-PAGE showed that the content of the protein with the molecular weight over Mr 74000 decreased, and the hybriditional signal in band M(r) 125000 weakened, while the band M(r)110000 and M(r)63000 strengthened in coccoid H.pylori as shown in Western blot. The results of PCR were all positive, and PCR-SSCP indicated that there may exist the point mutation in gene hpaA or vacA.
conclusionThe virulence and the proteins with molecular weight over M(r)74000 in coccoid H.pylori decrease, but no deletion exists in amplification fragments from ureA, ureB, hpaA, vacA and cagA genes, suggesting that coccoid H.pylori may have potential pathogenicity.
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