ArticleWorld journal of gastroenterology2001
HCV-RNA positivity in peripheral blood mononuclear cells of patients with chronic HCV infection: does it really mean viral replication?
Article in World journal of gastroenterology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 32 citations in OpenAlex.
- Detection of anti-HCV antibodies in the clinical classification and epidemiological surveillance of HCV infection.Molecular biology reports · 2025Review
- The blood transcriptional signature of chronic hepatitis C virus is consistent with an ongoing interferon-mediated antiviral response.Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research · 2013Article
- Analysis of a non-structural gene reveals evidence of possible hepatitis C virus (HCV) compartmentalization.Journal of medical virology · 2012Article
- Hepatitis C virus RNA quantitation in venous and capillary small-volume whole-blood samples.Journal of clinical microbiology · 2009Article
- Management of Hepatitis C in HIV-infected Patients.Current infectious disease reports · 2005Article
- Detection of micrometastasis of gastric carcinoma in peripheral blood circulation.World journal of gastroenterology · 2004Article
- Sequence evolution of putative cytotoxic T cell epitopes in NS3 region of hepatitis C virus.World journal of gastroenterology · 2004Article
- RNA interference: antiviral weapon and beyond.World journal of gastroenterology · 2003Review
- A small yeast RNA inhibits HCV IRES mediated translation and inhibits replication of poliovirus in vivo.World journal of gastroenterology · 2003Article
- HCV replication in PBMC and its influence on interferon therapy.World journal of gastroenterology · 2003Article
- Preparation of human single chain Fv antibody against hepatitis C virus E2 protein and its identification in immunohistochemistry.World journal of gastroenterology · 2002Article
- Genetic evolution of structural region of hepatitis C virus in primary infection.World journal of gastroenterology · 2002Article
- Identification of the epitopes on HCV core protein recognized by HLA-A2 restricted cytotoxic T lymphocytes.World journal of gastroenterology · 2001Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo analyze the association of HCV-RNA with peripheral blood mononuclear cells (PBMC) and to answer the question whether HCV-RNA positivity in PBMC is due to viral replication.
methodsHCV-RNA was monitored in serum and PBMC preparations from 15 patients with chronic HCV infection before, during and after an IFN-alpha therapy using a nested RT/PCR technique. In a second approach, PBMC from healthy donors were incubated in HCV positive plasma.
resultsIn the IFN-alpha responding patients,HCV-RNA disappeared first from total RNA preparations of PBMC and then from serum. In contrast, in relapsing patients, HCV-RNA reappeared first in serum and then in PBMC. A quantitative analysis of the HCV-RNA concentration in serum was performed before and after transition from detectable to non detectable HCV-RNA in PBMC-RNA and vice versa. When HCV-RNA was detectable in PBMC preparations, the HCV concentration in serum was significantly higher than the serum HCV-RNA concentration when HCV-RNA in PBMC was not detectable. Furthermore, at no time during the observation period was HCV specific RNA observed in PBMC, if HCV-RNA in serum was under the detection limit. Incubation of PBMC from healthy donors with several dilutions of HCV positive plasma for two hours showed a concentration dependent PCR positivity for HCV-RNA in reisolated PBMC.
conclusionThe detectability of HCV-RNA in total RNA from PBMC seems to depend on the HCV concentration in serum. Contamination or passive adsorption by circulating virus could be the reason for detection of HCV-RNA in PBMC preparations of chronically infected patients.
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